利用体外暴露法比较港湾海豹和灰海豹对几种环境污染物的敏感性。
Comparative sensitivity of harbour and grey seals to several environmental contaminants using in vitro exposure.
机构信息
Université du Québec à Montréal, Montréal, Québec H3C 3P8, Canada.
出版信息
Mar Pollut Bull. 2010 Mar;60(3):344-9. doi: 10.1016/j.marpolbul.2009.10.019. Epub 2009 Nov 28.
In this study, we investigated the effects of cadmium chloride (CdCl(2)), mercury chloride (HgCl(2)), methylmercury chloride (CH(3)HgCl), and PCBs on lymphocyte proliferation in phocids. PBMCs isolated from harbour and grey seals were exposed in vitro to varying concentrations of contaminants. A reduction of viability occurred when cells were exposed to 10(-4)M HgCl(2) or CH(3)HgCl or to 50ppm of Aroclor 1254. In both grey and harbour seals, T-lymphocyte proliferation was suppressed when their cells were incubated with 5 x 10(-5)M CdCl(2) or 10(-4)M HgCl(2). An inhibition of proliferation occurred with CH(3)HgCl from 10(-6)M in grey seals and from 10(-5)M in harbour seals. In grey seals, Aroclor 1254 reduced lymphocyte proliferation at 15ppm. In both harbour and grey seals, CH(3)HgCl was ten times more immunotoxic that HgCl(2). From IC(50), chemicals were ranked in terms of toxicity as followed: CH(3)HgCl>CdCl(2)>HgCl(2)>Aroclor 1254.
在这项研究中，我们研究了氯化镉（CdCl(2)）、氯化汞（HgCl(2)）、甲基汞氯化物（CH(3)HgCl）和多氯联苯（PCBs）对海豹淋巴细胞增殖的影响。从港湾海豹和灰海豹中分离出的 PBMC 在体外暴露于不同浓度的污染物中。当细胞暴露于 10(-4)M HgCl(2)或 CH(3)HgCl 或 50ppm Aroclor 1254 时，细胞活力降低。在灰海豹和港湾海豹中，当它们的细胞用 5 x 10(-5)M CdCl(2)或 10(-4)M HgCl(2)孵育时，T 淋巴细胞增殖受到抑制。CH(3)HgCl 从灰海豹的 10(-6)M 和港湾海豹的 10(-5)M 开始抑制增殖。在灰海豹中，Aroclor 1254 在 15ppm 时降低淋巴细胞增殖。在港湾海豹和灰海豹中，CH(3)HgCl 的免疫毒性是 HgCl(2)的十倍。根据 IC(50)，按毒性对化学品进行了排序：CH(3)HgCl>CdCl(2)>HgCl(2)>Aroclor 1254。
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