Design and evaluation of a fast Fourier transform-based nonlinear dielectric spectrometer.

Nonlinear dielectric spectroscopy of micro-organism is carried out by applying a moderate electrical field to an aqueous sample through two metal electrodes. Several ad hoc nonlinear spectrometers were proposed in the literature. However, these designs barely compensated the nonlinear distortion derived from the electrode-electrolyte interfaces (EEI). Moreover, the contribution of the suspension is masked by the effect of the nonlinearity introduced by the electrode contacts. Conversely, the nonlinear capability of a commercial tetrapolar analyzer has not been fully investigated. In this paper a new nonlinear tetrapolar spectrometer is proposed based on a commercial linear apparatus and ad hoc control and signal processing software. The system was evaluated with discrete electronic phantoms and showed that it can measure nonlinear properties of aqueous suspension independently of the presence of EEI (ANOVA test, p>0.001). It was also tested with real aqueous samples. The harmonics observed in the current that circulates through the sample reveals useful information about the transfer function of the sample. The total harmonic distortion was computed for linear mediums. Values lower than -60 dB suggest that the system has enough capability to perform nonlinear microbiological analysis. Design specifications, sources of interference, and equipment's limitations are discussed.