染料掺杂人工类脂膜的光电成像显微镜观察。
Electro-optical imaging microscopy of dye-doped artificial lipidic membranes.
机构信息
Laboratoire de Photonique Quantique et Moléculaire, Centre National de la Recherche Scientifique, UMR8537, d'Alembert Institute, Ecole Normale Supérieure de Cachan, Cachan, France.
出版信息
Biophys J. 2009 Dec 2;97(11):2913-21. doi: 10.1016/j.bpj.2009.08.055.
Artificial lipidic bilayers are widely used as a model for the lipid matrix in biological cell membranes. We use the Pockels electro-optical effect to investigate the properties of an artificial lipidic membrane doped with nonlinear molecules in the outer layer. We report here what is believed to be the first electro-optical Pockels signal and image from such a membrane. The electro-optical dephasing distribution within the membrane is imaged and the signal is shown to be linear as a function of the applied voltage. A theoretical analysis taking into account the statistical orientation distribution of the inserted dye molecules allows us to estimate the doped membrane nonlinearity. Ongoing extensions of this work to living cell membranes are discussed.
人工类脂双层膜被广泛用作生物细胞膜中脂基质的模型。我们利用电光 Pockels 效应研究了掺杂在外层中的非线性分子的人工类脂膜的性质。本文报道了据信是来自这种膜的第一个电光 Pockels 信号和图像。膜内电光退相分布被成像，并且信号显示为与所施加电压呈线性关系。考虑到插入染料分子的统计取向分布的理论分析使我们能够估计掺杂膜的非线性度。正在讨论将这项工作扩展到活细胞膜的问题。
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