从纤维蛋白原纯化上清液中分离出巴西矛头蝮蛇抗凝血酶。
Isolation of Bothrops jararaca snake antithrombin from the supernatant of fibrinogen purification.
作者信息
Morais-Zani K, Tanaka A S, Tanaka-Azevedo A M
机构信息
Laboratory of Pathophysiology, Butantan Institute, São Paulo, Brazil.
出版信息
J Biomol Tech. 2009 Dec;20(5):249-52.
Abstract
A novel method of antithrombin (AT) purification from Bothrops jararaca snake plasma was developed to obtain this protein using a waste supernatant from B. jararaca fibrinogen purification. The AT purification was achieved by affinity chromatography on HiTrap Heparin HP. The results showed an efficient purification process yielding pure AT (purity 65-fold and specific activity 368.91). In conclusion, we showed a feasible purification method of AT from B. jararaca plasma using a discarded material. This feature is important, considering the limitation of material, such as snake plasma, and could also be useful to obtain pure plasma proteins from other animals, including human plasma.
摘要
开发了一种从巴西矛头蝮蛇血浆中纯化抗凝血酶（AT）的新方法，该方法利用巴西矛头蝮纤维蛋白原纯化过程中的废弃上清液来获取这种蛋白质。通过在HiTrap Heparin HP上进行亲和层析实现了AT的纯化。结果显示该纯化过程效率高，可产生纯的AT（纯度提高65倍，比活性为368.91）。总之，我们展示了一种利用废弃材料从巴西矛头蝮血浆中纯化AT的可行方法。考虑到诸如蛇血浆等材料的局限性，这一特性很重要，并且对于从包括人血浆在内的其他动物中获取纯血浆蛋白也可能有用。
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