Ma Chao, Xie Jia-Wei, Kuang An-Ren, Huang Rui, Tang Gong-Shun
Department of Nuclear Medicine, West China Hospital, Sichuan University, Chengdu, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 Sep;40(5):780-3.
To evaluate the effects of phosphorothioate antisense oligonucleotides (ASON) bcl-2/ bc-xl ASON and bcl-2 on the proliferation and apoptosis of breast cancer cells, MCF-7.
The significant difference of nuclear condensation, chromatin fragmentation and apoptotic bodies in MCF-7 cells, typical of apoptotic cell death was observed in groups of bcl-2/ bcl-xl bispecific ASON by compared with that of bcl-2 ASON treatment. The fluorensence intensities of bcl-2 in groups of NA-D and NA-S, PA-S and PA-D, FB1 and FB2 were 1.92+/-0.08 and 2.83+/-0.16 (P=0.028); 4.20+/-0.18 and 2.85+/-0.57 (P=0.001); 5.70+/-1.16 and 4.35+/-0.11 (P=0.001), respectively. The cell survival rates at 24 h of NA-D and NA-S, PA-S and PA-D, FB1 and FB2 were (0.32+/-0.03)% and (0.58+/-0.07)% (P=0.014); (0.71+/-0.03)% and (0.45+/-0.04)% (P=0.014); (0.88+/-0.04)% and (0.57+/- 0.05)% (P=0.003), respectively.
The bcl-2/bct-xl bispecific ASON could inhibit bcl-2 expression and induce apoptosis of breast cancer cells more efficiently than that treated with bcl-2 ASON alone.
评估硫代磷酸酯反义寡核苷酸（ASON）bcl-2/bc-xl ASON和bcl-2对乳腺癌细胞MCF-7增殖和凋亡的影响。
1）分别用阴离子型长循环脂质体（NA）、阳离子型脂质体（PA）将bcl-2 ASON和bcl-2/bcl-xl ASON转染至MCF-7细胞。2）用bcl-2 ASON（FB1）、bcl-2/bcl-xl ASON（FB2）、负载bcl-2 ASON的NA（NA-S）或bcl-2/bcl-xl ASON（NA-D）、负载bcl-2 ASON的PA（PA-S）或bcl-2/bc-xl ASON（PA-D）孵育24小时后，通过苏木精-伊红（HE）染色、甲基噻唑基四氮唑（MTT）法和流式细胞术（FCM）评估它们对MCF-7细胞的抑制作用。
与bcl-2 ASON处理组相比，bcl-2/bcl-xl双特异性ASON组中观察到MCF-7细胞出现典型凋亡性细胞死亡的核固缩、染色质碎片化和凋亡小体的显著差异。NA-D和NA-S组、PA-S和PA-D组、FB1和FB2组中bcl-2的荧光强度分别为1.92±0.08和2.83±0.16（P = 0.028）；4.20±0.18和2.85±0.57（P = 0.001）；5.70±1.16和4.35±0.11（P = 0.001）。NA-D和NA-S组、PA-S和PA-D组、FB1和FB2组在24小时时的细胞存活率分别为（0.32±0.03）%和（0.58±0.07）%（P = 0.014）；（0.71±0.03）%和（0.45±0.04）%（P = 0.014）；（0.88±0.04）%和（0.57±0.05）%（P = 0.003）。
bcl-2/bct-xl双特异性ASON比单独使用bcl-2 ASON能更有效地抑制bcl-2表达并诱导乳腺癌细胞凋亡。
Zotero 插件