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利用高通量 RNAi 策略洞察转录网络。

Insights to transcriptional networks by using high throughput RNAi strategies.

机构信息

Merck Research Laboratories, Department of Molecular Profiling-Research Informatics, Rahway, NJ 07065, USA.

出版信息

Brief Funct Genomics. 2010 Jan;9(1):43-52. doi: 10.1093/bfgp/elp046. Epub 2009 Dec 1.

DOI:10.1093/bfgp/elp046
PMID:19952073
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3097102/
Abstract

RNA interference (RNAi) is a powerful method to unravel the role of a given gene in eukaryotic cells. The development of high throughput assay platforms such as fluorescence plate readers and high throughput microscopy has allowed the design of genome wide RNAi screens to systemically discern members of regulatory networks around various cellular processes. Here we summarize the different strategies employed in RNAi screens to reveal regulators of transcriptional networks. We focus our discussion in experimental approaches designed to uncover regulatory interactions modulating transcription factor activity.

摘要

RNA 干扰 (RNAi) 是一种强大的方法，可以揭示真核细胞中特定基因的作用。高通量检测平台（如荧光板读数器和高通量显微镜）的发展，使得设计全基因组 RNAi 筛选成为可能，从而系统性地辨别各种细胞过程的调控网络中的成员。在这里，我们总结了 RNAi 筛选中用于揭示转录网络调节因子的不同策略。我们的讨论重点是设计用于揭示调节转录因子活性的调节相互作用的实验方法。

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本文引用的文献

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Functional genomic approaches targeting the wnt signaling network.针对Wnt信号网络的功能基因组学方法。
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2
Bili inhibits Wnt/beta-catenin signaling by regulating the recruitment of axin to LRP6.胆红素通过调节轴蛋白向低密度脂蛋白受体相关蛋白6（LRP6）的募集来抑制Wnt/β-连环蛋白信号通路。
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A network-based integrative approach to prioritize reliable hits from multiple genome-wide RNAi screens in Drosophila.一种基于网络的综合方法，用于在果蝇的多个全基因组RNA干扰筛选中确定可靠的命中结果。
BMC Genomics. 2009 May 12;10:220. doi: 10.1186/1471-2164-10-220.
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Systems-wide analysis of a phosphatase knock-down by quantitative proteomics and phosphoproteomics.通过定量蛋白质组学和磷酸化蛋白质组学对磷酸酶敲低的系统分析。
Mol Cell Proteomics. 2009 Aug;8(8):1908-20. doi: 10.1074/mcp.M800559-MCP200. Epub 2009 May 9.
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Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.通过转基因RNA干扰对果蝇Notch信号通路进行全基因组分析。
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A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity.一项针对Oct4调节因子的全基因组RNA干扰筛选确定了Paf1复合物在胚胎干细胞特性维持中的作用。
Cell Stem Cell. 2009 May 8;4(5):403-15. doi: 10.1016/j.stem.2009.03.009. Epub 2009 Apr 2.
7
A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal.全基因组RNA干扰筛选鉴定出自我更新所需的一个新转录模块。
Genes Dev. 2009 Apr 1;23(7):837-48. doi: 10.1101/gad.1769609.
8
Using multiplexed regulation of luciferase activity and GFP translocation to screen for FOXO modulators.利用荧光素酶活性和绿色荧光蛋白转位的多重调控筛选FOXO调节剂。
BMC Cell Biol. 2009 Feb 25;10:14. doi: 10.1186/1471-2121-10-14.
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J Clin Pathol. 2009 Mar;62(3):195-200. doi: 10.1136/jcp.2008.058735. Epub 2009 Jan 6.
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RNAi screening for kinases and phosphatases identifies FoxO regulators.针对激酶和磷酸酶的RNA干扰筛选鉴定出FoxO调节因子。
Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):14873-8. doi: 10.1073/pnas.0803022105. Epub 2008 Sep 24.

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