Merck Research Laboratories, Department of Molecular Profiling-Research Informatics, Rahway, NJ 07065, USA.
Brief Funct Genomics. 2010 Jan;9(1):43-52. doi: 10.1093/bfgp/elp046. Epub 2009 Dec 1.
RNA interference (RNAi) is a powerful method to unravel the role of a given gene in eukaryotic cells. The development of high throughput assay platforms such as fluorescence plate readers and high throughput microscopy has allowed the design of genome wide RNAi screens to systemically discern members of regulatory networks around various cellular processes. Here we summarize the different strategies employed in RNAi screens to reveal regulators of transcriptional networks. We focus our discussion in experimental approaches designed to uncover regulatory interactions modulating transcription factor activity.
RNA 干扰 (RNAi) 是一种强大的方法，可以揭示真核细胞中特定基因的作用。高通量检测平台（如荧光板读数器和高通量显微镜）的发展，使得设计全基因组 RNAi 筛选成为可能，从而系统性地辨别各种细胞过程的调控网络中的成员。在这里，我们总结了 RNAi 筛选中用于揭示转录网络调节因子的不同策略。我们的讨论重点是设计用于揭示调节转录因子活性的调节相互作用的实验方法。
