Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, the Netherlands.
Biotechnol Bioeng. 2010 Apr 1;105(5):918-23. doi: 10.1002/bit.22612.
Phosphate removal to ecologically desired levels of <0.01 mg/L is currently dependent on large overdosing of metal salts and production of large amounts of chemical sludge. The present study focuses on the development and performance of a new bionanotechnological phosphate removal system, based on sorption of oxoanions by nanoscale ferric iron particles stabilized within thermostable ferritin from the hyperthermophilic archaeon Pyrococcus furiosus (PfFrt). Laboratory studies show that this thermostable protein nanocage has fast kinetics for phosphate uptake at very low concentrations by catalytic oxidation of iron. In this study we demonstrate essentially complete phosphate removal with a capacity of approximately 11 mg/g PfFrt. Ferritin can easily be immobilized and is amenable to fast and efficient regeneration, making recovery of phosphate possible. The phosphate removal process with PfFrt is, due to its high affinity, able to reach ecologically desired phosphate levels and in addition it is cost competitive with existing techniques.
目前，将磷酸盐去除到生态所需的 <0.01 毫克/升的水平依赖于大量金属盐的过量投加和大量化学污泥的产生。本研究专注于开发和展示一种新的基于纳米尺度的铁颗粒的生物纳米技术磷酸盐去除系统，该系统通过嗜热古菌 Pyrococcus furiosus（PfFrt）中的热稳定铁蛋白稳定的氧阴离子吸附来实现。实验室研究表明，这种热稳定蛋白纳米笼具有快速的动力学特性，可通过铁的催化氧化在非常低的浓度下吸收磷酸盐。在本研究中，我们证明了 PfFrt 的磷去除容量约为 11mg/g，几乎可以完全去除磷。铁蛋白易于固定，并且易于快速高效地再生，从而实现了磷酸盐的回收。由于 PfFrt 具有高亲和力，因此磷去除过程能够达到生态所需的磷水平，此外，它在成本上也具有竞争力，优于现有技术。
