Scott D, McLaren A, Dyson J, Simpson E
Transplantation Biology, Clinical Research Centre, Harrow, Middlesex, England.
Immunogenetics. 1991;33(1):54-61. doi: 10.1007/BF00211696.
Cloned B-cell lines from a female T16H/XSxr mouse in which Tdy expression was suppressed due to X inactivation and from a male X/XSxr mouse, both of the (kxb)F1 haplotype, were examined for H-Y expression. This was determined both by their ability to act as targets for H-2k and H-2b-restricted H-Y-specific cytotoxic T cells and by their ability to stimulate the proliferation of H-2Kk, H-2Db (class I) and Ab (class II)-restricted T-cell clones. In B-cell clones from the T16H/XSxr mouse, expression of H-Y/Db exhibited partial X inactivation and only a proportion (congruent to 30%) of the cells were targets for or stimulated H-2Db-restricted H-Y-specific T cells. In contrast, H-Y epitopes restricted by H-2k (H-Y/Kk, H-Y/Dk) and Ab (H-Y/Ab) exhibited no X inactivation. Furthermore, no inactivation of H-Y/Db, H-Y/Ab, or H-Yk was observed in the male X/XSxr mouse. These results indicate that the T16H/XSxr female is a mosaic, as a result of the variable spread of X inactivation into the Sxr region. They further suggest that the H-Y antigen recognized in association with H-2k and H-2Db class I molecules and Ab class II molecules may be the product of more than one gene.
从一只雌性T16H/XSxr小鼠（由于X染色体失活，Tdy表达受到抑制）和一只雄性X/XSxr小鼠（两者均为(kxb)F1单倍型）克隆得到的B细胞系，被检测了H-Y表达情况。这通过它们作为H-2k和H-2b限制性H-Y特异性细胞毒性T细胞靶标的能力以及刺激H-2Kk、H-2Db（I类）和Ab（II类）限制性T细胞克隆增殖的能力来确定。在来自T16H/XSxr小鼠的B细胞克隆中，H-Y/Db的表达表现出部分X染色体失活，只有一部分（约30%）细胞是H-2Db限制性H-Y特异性T细胞的靶标或能刺激这些T细胞。相比之下，受H-2k（H-Y/Kk、H-Y/Dk）和Ab（H-Y/Ab）限制的H-Y表位未表现出X染色体失活。此外，在雄性X/XSxr小鼠中未观察到H-Y/Db、H-Y/Ab或H-Yk的失活。这些结果表明，由于X染色体失活向Sxr区域的可变扩展，T16H/XSxr雌性小鼠是一个嵌合体。它们还进一步表明，与H-2k和H-2Db I类分子以及Ab II类分子相关识别的H-Y抗原可能是多个基因的产物。
