血小板激活因子在视网膜色素上皮细胞和脉络膜内皮细胞中的功能分析。
Functional analysis of platelet-activating factor in the retinal pigment epithelial cells and choroidal endothelial cells.
机构信息
Department of Ophthalmology, UT Southwestern Medical Center at Dallas, Dallas, Texas 75390-9057, USA.
出版信息
Curr Eye Res. 2009 Nov;34(11):957-65. doi: 10.3109/02713680903231135.
PURPOSE
To investigate the function of platelet-activating factor (PAF) in cultured retinal pigment epithelial (RPE) and choroidal endothelial (CE) cells.
METHODS
The in vitro and in vivo expression of PAF-receptors (PAF-R) on both these cells was determined. The production of PAF by RPE cells was also determined. The effect of PAF on the proliferation, migration, permeability, and apoptosis of CE cells was examined, and the modulation of PAF on the VEGF level in RPE cells was assessed.
RESULTS
PAF-R was present in both types of cells in vitro, as well as in RPE and choroid in vivo. Cultured RPE cells synthesized PAF. PAF stimulated CE cell migration and permeability but not the proliferation. PAF also increased the VEGF level in RPE cells.
CONCLUSIONS
Similar to VEGF, PAF stimulates CE cell migration and permeability. It also up-regulates VEGF level in RPE cells. PAF may be involved in the pathogenesis of choroidal neovascularization.
目的
研究血小板激活因子（PAF）在培养的视网膜色素上皮（RPE）和脉络膜内皮（CE）细胞中的作用。
方法
测定这两种细胞上 PAF 受体（PAF-R）的体外和体内表达情况。还测定了 RPE 细胞产生 PAF 的情况。研究了 PAF 对 CE 细胞增殖、迁移、通透性和凋亡的影响，并评估了 PAF 对 RPE 细胞中 VEGF 水平的调节作用。
结果
PAF-R 存在于体外的两种细胞以及体内的 RPE 和脉络膜中。培养的 RPE 细胞合成 PAF。PAF 刺激 CE 细胞迁移和通透性，但不刺激增殖。PAF 还增加了 RPE 细胞中的 VEGF 水平。
结论
与 VEGF 类似，PAF 刺激 CE 细胞迁移和通透性。它还上调了 RPE 细胞中的 VEGF 水平。PAF 可能参与脉络膜新生血管的发病机制。
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