rpsL 在伯氏疏螺旋体中的反选择标记应用。
Use of rpsL as a Counterselectable Marker in Borrelia burgdorferi.
机构信息
Division of Biological Sciences, The University of Montana, Missoula, MT 59812-4824, USA.
出版信息
Appl Environ Microbiol. 2010 Feb;76(3):985-7. doi: 10.1128/AEM.02172-09. Epub 2009 Dec 4.
Abstract
We have demonstrated that rpsL, encoding the S12 protein of the small ribosomal subunit, can be used as a counterselectable marker in Borrelia burgdorferi, the causative agent of Lyme disease. Mutations in rpsL confer streptomycin resistance. Streptomycin susceptibility is dominant in an rpsL merodiploid, and streptomycin selects for the loss of wild-type rpsL carried in trans. This is the first description of a counterselectable marker in B. burgdorferi.
摘要
我们已经证明，编码小核糖体亚基 S12 蛋白的 rpsL 可作为莱姆病病原体伯氏疏螺旋体的可反向选择标记。rpsL 突变赋予链霉素抗性。rpsL 部分二倍体中链霉素敏感性是显性的，而链霉素选择反式携带的野生型 rpsL 的缺失。这是 B. burgdorferi 中第一个可反向选择标记的描述。
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