Watanabe Nobuhisa, Takasaki Yozo, Sato Chika, Ando Shoji, Tanaka Isao
Department of Biotechnology and Biomaterial Chemistry, Graduate School of Engineering, Nagoya University, Japan.
Acta Crystallogr D Biol Crystallogr. 2009 Dec;65(Pt 12):1326-33. doi: 10.1107/S0907444909041134. Epub 2009 Nov 17.
The three-dimensional crystal structures of HindIII bound to its cognate DNA with and without divalent cations were solved at 2.17 and 2.00 A resolution, respectively. HindIII forms a dimer. The structures showed that HindIII belongs to the EcoRI-like (alpha-class) subfamily of type II restriction endonucleases. The cognate DNA-complex structures revealed the specific DNA-recognition mechanism of HindIII by which it recognizes the palindromic sequence A/AGCTT. In the Mg(2+) ion-soaked structure the DNA was cleaved and two ions were bound at each active site, corresponding to the two-metal-ion mechanism.
分别以2.17埃和2.00埃的分辨率解析了结合同源DNA且有或无二价阳离子的HindIII的三维晶体结构。HindIII形成二聚体。这些结构表明，HindIII属于II型限制性内切酶的EcoRI样（α类）亚家族。同源DNA复合物结构揭示了HindIII识别回文序列A/AGCTT的特定DNA识别机制。在镁离子浸泡结构中，DNA被切割，每个活性位点结合两个离子，这与双金属离子机制相对应。
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