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澳链尾蝎毒液中一种新型杀虫毒素LaIT2的纯化及cDNA克隆

Purification and cDNA cloning of LaIT2, a novel insecticidal toxin from venom of the scorpion Liocheles australasiae.

作者信息

Matsushita Nobuto, Miyashita Masahiro, Ichiki Yayoi, Ogura Takehiko, Sakuradani Eiji, Nakagawa Yoshiaki, Shimizu Sakayu, Miyagawa Hisashi

机构信息

Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.

出版信息

Biosci Biotechnol Biochem. 2009 Dec;73(12):2769-72. doi: 10.1271/bbb.90509. Epub 2009 Dec 7.

DOI:10.1271/bbb.90509
PMID:19966481
Abstract

The novel insecticidal toxin, LaIT2, was isolated from venom of the scorpion Liocheles australasiae. The amino acid sequence of LaIT2 was determined by an Edman degradation analysis and subsequent cDNA cloning. LaIT2 is composed of 59 amino acids with three disulfide bridges, and shares sequence similarity to the scorpion beta-KTx peptides.

摘要

新型杀虫毒素LaIT2是从澳链尾蝎的毒液中分离出来的。通过埃德曼降解分析和随后的cDNA克隆确定了LaIT2的氨基酸序列。LaIT2由59个氨基酸组成，有三个二硫键，与蝎β-KTx肽具有序列相似性。

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