巨噬细胞移动抑制因子在小鼠泪腺中的高表达。

High expression of macrophage migration inhibitory factor in the murine lacrimal gland.

机构信息

Department of Ophthalmology, Johns Hopkins University, Baltimore, MD 21117, USA.

出版信息

Cornea. 2010 Feb;29(2):187-91. doi: 10.1097/ICO.0b013e3181c06466.

DOI:10.1097/ICO.0b013e3181c06466

PMID:19966563

Abstract

PURPOSE

To report the expression of macrophage migration inhibitory factor (MIF) in murine lacrimal glands.

METHODS

Six lacrimal glands from 3 female 8-week-old CBA/J mice were analyzed using a gene microarray method (Oligo GEArray mouse inflammatory cytokines and receptors, Catalog No. OMM-011, SuperArray Bioscience) and immunofluorescent staining for MIF. Nine lacrimal glands from 8-week-old CBA/J mice, 9 lacrimal glands from 8-week-old C57BL/6 mice, and 7 lacrimal glands from 8-week-old BALB/c mice were analyzed using real-time reverse transcription-polymerase chain reaction. Five spleens, 5 livers, and 5 serum samples from 5 female 8-week-old CBA/J mice were analyzed using enzyme-linked immunosorbent assay.

RESULTS

Microarray analysis revealed that MIF is highly expressed in the murine lacrimal gland. Lacrimal acinar cells stain strongly with anti-MIF antibodies. Real-time reverse transcription-polymerase chain reaction revealed that the MIF mRNA expression level is lower in lacrimal glands of CBA/J mice compared with C57BL/6 and BALB/c mice when normalized against the expression of beta-actin mRNA. Enzyme-linked immunosorbent assay revealed that MIF level was higher in lacrimal glands and spleens compared with livers and sera.

CONCLUSION

The murine lacrimal gland expresses high levels of macrophage MIF without any evidence of lacrimal gland inflammation.

摘要

目的

报告巨噬细胞移动抑制因子（MIF）在小鼠泪腺中的表达。

方法

采用基因微阵列法（Oligo GEArray 小鼠炎症细胞因子和受体，目录号 OMM-011，SuperArray Bioscience）和 MIF 免疫荧光染色分析 3 只 8 周龄 CBA/J 雌性小鼠的 6 只泪腺。用实时逆转录-聚合酶链反应分析 8 周龄 CBA/J 小鼠的 9 只泪腺、8 周龄 C57BL/6 小鼠的 9 只泪腺和 8 周龄 BALB/c 小鼠的 7 只泪腺。用酶联免疫吸附试验分析 5 只 8 周龄 CBA/J 雌性小鼠的 5 个脾脏、5 个肝脏和 5 个血清样本。

结果

微阵列分析显示 MIF 在小鼠泪腺中高度表达。抗 MIF 抗体对泪腺上皮细胞染色强烈。实时逆转录-聚合酶链反应显示，与 C57BL/6 和 BALB/c 小鼠相比，CBA/J 小鼠泪腺 MIF mRNA 的表达水平在正常化到β-肌动蛋白 mRNA 的表达后较低。酶联免疫吸附试验显示，MIF 水平在泪腺和脾脏中高于肝脏和血清。