Department of Clinical Chemistry and Molecular Diagnostics, Philipps-University of Marburg, 35033 Marburg, Germany.
J Allergy Clin Immunol. 2010 Jan;125(1):108-15.e1-3. doi: 10.1016/j.jaci.2009.09.019. Epub 2009 Dec 6.
Traditional farming represents a unique model situation to investigate the relationship of early-life farm-related exposure and allergy protection.
To investigate associations between maternal farm exposures and cytokine production in cord blood (CB) mononuclear cells in a prospective multinational birth cohort of 299 farm and 326 nonfarm children and their families.
Supernatants from phorbol 12-myristate 13-acetate/ionomycin-stimulated CB mononuclear cells were assessed for the production of IFN-gamma, TNF-alpha, IL-5, IL-10, and IL-12.
Significantly higher levels of IFN-gamma and TNF-alpha in farm compared with nonfarm children were found, whereas IL-5, IL-10, and IL-12 levels did not differ between study groups. Maternal contact with different farm animal species and barns and consumption of farm-produced butter during pregnancy enhanced the production of proinflammatory CB cytokines, whereas maternal consumption of farm-produced yogurt resulted in significant lower levels of IFN-gamma and TNF-alpha in umbilical blood.
Maternal exposure to farming activities and farm dairy products during pregnancy modulated cytokine production patterns of offspring at birth.
传统农业代表了一种独特的模式，可以用来研究生命早期与农场相关的暴露与过敏保护之间的关系。
在一个由 299 名农场儿童和 326 名非农场儿童及其家庭组成的前瞻性跨国出生队列中，调查母体农场暴露与脐血（CB）单个核细胞细胞因子产生之间的关联。
用佛波醇 12-肉豆蔻酸 13-乙酸酯/离子霉素刺激 CB 单个核细胞，评估 IFN-γ、TNF-α、IL-5、IL-10 和 IL-12 的产生。
与非农场儿童相比，农场儿童的 IFN-γ 和 TNF-α水平明显升高，而两组间 IL-5、IL-10 和 IL-12 水平无差异。母亲在怀孕期间接触不同的农场动物种类和畜棚，以及食用农场生产的黄油，会增强促炎 CB 细胞因子的产生，而母亲食用农场生产的酸奶会导致脐血中 IFN-γ 和 TNF-α水平显著降低。
母体在怀孕期间接触农场活动和农场乳制品会调节后代出生时的细胞因子产生模式。
