在具有嘌呤 - 嘧啶交替序列的多核苷酸中独特B结构的存在。

The existence of unique B structures in polynucleotides with alternating purine-pyrimidine sequences.

作者信息

Loprete D M, Hartman K A

机构信息

Department of Biochemistry and Biophysics, University of Rhode Island, Kingston 02881.

出版信息

Biochem Biophys Res Commun. 1991 Feb 14;174(3):1313-7. doi: 10.1016/0006-291x(91)91565-t.

DOI:10.1016/0006-291x(91)91565-t

PMID:1996995

Abstract

The infrared spectra of the sodium salts of calf-thymus DNA, poly(dA-dC).poly(dG-dT), poly(dA-dT) and poly(dG-dC) were measured for the samples as highly hydrated, nonoriented gels. The bands from the sugar-phosphate vibrational modes show that poly(dG-dC) assumes a B-family structure which is different from the B structures of the other samples. Poly(dG-dC) most likely assumes a wrinkled B structure. The other samples retain a smooth B structure. An alternating purine-pyrimidine sequence is not a sufficient condition for the formation of wrinkled B structure in a polynucleotide.

摘要

对高度水合的非定向凝胶状小牛胸腺DNA、聚（dA-dC）·聚（dG-dT）、聚（dA-dT）和聚（dG-dC）的钠盐进行了红外光谱测量。糖-磷酸振动模式产生的谱带表明，聚（dG-dC）呈现出一种B族结构，与其他样品的B结构不同。聚（dG-dC）很可能呈现出一种有褶皱的B结构。其他样品则保持光滑的B结构。嘌呤-嘧啶交替序列不是多核苷酸中形成有褶皱B结构的充分条件。