Stanislawski L, Sorin M
Institut National de la Santé et de la Recherche Médicale (U.294), CHU 7 Xavier Bichat, Université Paris 7, France.
Infect Immun. 1991 Mar;59(3):949-54. doi: 10.1128/iai.59.3.949-954.1991.
Recurrent pulmonary infections with Staphylococcus aureus and Pseudomonas aeruginosa are a major problem in cystic fibrosis (CF), leading to severe and progressive deterioration of the respiratory tract. Fibronectin (FN) has a binding site(s) for S. aureus and Streptococcus pyogenes. This bacterial bindings site(s) was studied by using FN from patients with CF and comparing them with FN purified from the plasma of bronchitis and normal subjects by an enzyme-linked immunosorbent assay technique. We found a decreased binding of the CF FN to these bacteria in all seven CF patients. Other differences between the CF FN and normal FN were a marked modification in the pattern of peptide migration in polyacrylamide gel electrophoresis after hydrolysis with various proteinases and an increase or decrease of the total sugar content of the FN.
金黄色葡萄球菌和铜绿假单胞菌引起的复发性肺部感染是囊性纤维化（CF）的一个主要问题，会导致呼吸道严重且进行性恶化。纤连蛋白（FN）有一个针对金黄色葡萄球菌和化脓性链球菌的结合位点。通过使用CF患者的FN，并将其与通过酶联免疫吸附测定技术从支气管炎患者血浆和正常受试者血浆中纯化得到的FN进行比较，对该细菌结合位点进行了研究。我们发现，在所有7例CF患者中，CF FN与这些细菌的结合均减少。CF FN与正常FN之间的其他差异包括，在用各种蛋白酶水解后，聚丙烯酰胺凝胶电泳中肽迁移模式有明显改变，以及FN总糖含量增加或减少。
