小鼠淋巴样细胞系有丝分裂细胞中聚乙二醇介导的融合能力增强。

Increased polyethylene glycol-mediated fusion competence in mitotic cells of a mouse lymphoid cell line.

作者信息

Hansen D, Stadler J

出版信息

Somatic Cell Genet. 1977 Sep;3(5):471-82. doi: 10.1007/BF01539119.

PMID:199953

Abstract

Spontaneous mitotic cells of the mouse leukemic cell line GF7 are preferentially included in cell fusion products after treatment with polyethylene glycol (PEG). This implies a unique configuration of the natural mitotic membrane which is particularly vulnerable to induction of fusion by PEG. Colcemid-arrested GF7 mitotic cells, however, are excluded from PEG-induced cell fusion products, suggesting that colcemid reverses the membrane configuration which is susceptible to the action of PEG. When Sendai virus is used as the fusogenic agent, both colcemid-arrested and spontaneous mitotic cells are selectively fused. There must, therefore, be an essential membrane-fusogen reaction which is characteristically different for each of these agents.

摘要

用聚乙二醇（PEG）处理后，小鼠白血病细胞系GF7的自发有丝分裂细胞优先被纳入细胞融合产物中。这意味着天然有丝分裂膜具有独特的结构，特别容易受到PEG诱导融合的影响。然而，秋水仙酰胺阻滞的GF7有丝分裂细胞被排除在PEG诱导的细胞融合产物之外，这表明秋水仙酰胺可逆转易受PEG作用影响的膜结构。当仙台病毒用作融合剂时，秋水仙酰胺阻滞的和自发有丝分裂细胞都会被选择性融合。因此，必然存在一种基本的膜融合剂反应，每种融合剂的反应特性都不同。