Van den Berg C W, Aerts P C, Van Dijk H
Eijkman-Winkler Laboratory of Medical Microbiology, Department of Experimental Microbiology, Faculty of Medicine, University of Utrecht, The Netherlands.
J Immunol Methods. 1991 Feb 15;136(2):287-94. doi: 10.1016/0022-1759(91)90015-8.
The kinetics of complement (C) depletion and recovery of C levels upon injection of BALB/c mice with cobra venom factors (CVF), from N. naja (C3- and C5-depleting) and N. haje (selectively C3-depleting) were studied. The animals received i.p. or i.v. injections of either of the two preparations. CH50 and hemolytic C3 and C5 levels were followed as parameters of residual complement activity. N. naja CVF turned out to be as efficient in depleting total complement activity as N. haje CVF. Decreased CH50 values could largely be ascribed to C3 depletion. Complement consumption after N. naja CVF, however, lasted longer than after N. haje CVF administration. Estimated functional half-lives of N. naja and N. haje CVF were 11.5 and 4.5 h, respectively. Inhibition ELISAs showed that, after in vivo administration of either of the two CVF preparations, antigenic C3 and C5 kept circulating for days.
研究了用眼镜蛇毒因子（CVF）注射BALB/c小鼠后补体（C）消耗的动力学以及C水平的恢复情况，所用CVF分别来自眼镜蛇（能消耗C3和C5）和埃及眼镜蛇（选择性消耗C3）。动物通过腹腔注射或静脉注射两种制剂中的任一种。跟踪CH50以及溶血C3和C5水平作为残余补体活性的参数。结果表明，眼镜蛇CVF在消耗总补体活性方面与埃及眼镜蛇CVF一样有效。CH50值降低很大程度上归因于C3的消耗。然而，眼镜蛇CVF后的补体消耗持续时间比埃及眼镜蛇CVF给药后更长。眼镜蛇和埃及眼镜蛇CVF的估计功能半衰期分别为11.5小时和4.5小时。抑制性酶联免疫吸附测定表明，在体内给予两种CVF制剂中的任一种后，抗原性C3和C5会持续循环数天。
