Soares Anna L, Fernandes Ana P, Cardoso Jarbas E, Sousa Marinez O, Lasmar Marcelo C, Novelli Bethânia A, Lages Geralda F, Dusse Luci M, Vieira Lauro M, Lwaleed Bashir A, Carvalho Maria G
Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Pathophysiol Haemost Thromb. 2008;36(5):275-81. doi: 10.1159/000252825. Epub 2009 Dec 9.
Thrombotic episodes account for approximately 80% of deaths in type 2 diabetic patients. Hyperhomocysteinaemia is a well recognized independent risk factor for atherosclerosis and thromboembolism. Increased homocysteine levels may occur due to a number of factors including inherited gene polymorphism of methylenetetrahydrofolate reductase (MTHFR) C677T. Here, we evaluate plas- ma total homocysteine (tHcy) levels and frequency of the MTHFR C677T gene polymorphism in asymptomatic healthy volunteers and type 2 diabetic patients with hypertension but without nephropathy. We have also investigated the relationship between tHcy levels and the presence of MTHFR C677T gene polymorphism.
Plasma tHcy levels and MTHFR C677T genotype were investigated in a total of 53 subjects. These included asymptomatic healthy volunteers (n = 16), patients with type 2 diabetes (n = 7), subjects with hypertension (n = 12) and patients with both type 2 diabetes and hypertension (n = 18). Renal function, serum lipids and other metabolites were also assessed.
There was no significant difference in tHcy levels between the groups studied. The frequency of MTHFR C677T gene polymorphism observed was similar to that obtained for the general Brazilian population. In patients with type 2 diabetes and hypertension but without impaired renal function, we observed no meaningful correlation between increased tHcy levels and the presence of MTHFR C677T gene polymorphism.
Type 2 diabetics who are homozygous or heterozygous for the MTHFR C677T gene polymorphism showed normal tHcy levels. Our results further suggest that diabetes without an associated adverse risk profile is not an independent correlate of increased tHcy levels.
血栓形成事件约占2型糖尿病患者死亡人数的80%。高同型半胱氨酸血症是动脉粥样硬化和血栓栓塞公认的独立危险因素。同型半胱氨酸水平升高可能由多种因素引起,包括亚甲基四氢叶酸还原酶(MTHFR)C677T的遗传基因多态性。在此,我们评估无症状健康志愿者以及患有高血压但无肾病的2型糖尿病患者的血浆总同型半胱氨酸(tHcy)水平和MTHFR C677T基因多态性频率。我们还研究了tHcy水平与MTHFR C677T基因多态性之间的关系。
共对53名受试者的血浆tHcy水平和MTHFR C677T基因型进行了研究。这些受试者包括无症状健康志愿者(n = 16)、2型糖尿病患者(n = 7)、高血压患者(n = 12)以及同时患有2型糖尿病和高血压的患者(n = 18)。还评估了肾功能、血脂和其他代谢产物。
所研究的各组之间tHcy水平无显著差异。观察到的MTHFR C677T基因多态性频率与巴西普通人群的频率相似。在患有2型糖尿病和高血压但肾功能未受损的患者中,我们未观察到tHcy水平升高与MTHFR C677T基因多态性之间存在有意义的相关性。
MTHFR C677T基因多态性纯合或杂合的2型糖尿病患者tHcy水平正常。我们的结果进一步表明,无相关不良风险特征的糖尿病并非tHcy水平升高的独立相关因素。