药物 - DNA 系统的圆二色性和线性二色性。

Circular and linear dichroism of drug-DNA systems.

作者信息

Rodger Alison

机构信息

Department of Chemistry, University of Warwick, Coventry, UK.

出版信息

Methods Mol Biol. 2010;613:37-54. doi: 10.1007/978-1-60327-418-0_3.

DOI:10.1007/978-1-60327-418-0_3

PMID:19997876

Abstract

When a drug binds to DNA, its electronic structure is perturbed, and it perturbs the DNA's electronic structure. The resulting change to the electronic spectroscopy can be used to probe the drug-DNA interaction. This chapter outlines how circular and linear dichroism spectroscopy can be used to provide information about drug-DNA systems. Circular dichroism spectroscopy involves measuring the difference in absorption of left and right circularly polarized light. It is uniquely sensitive to the helicity of the molecules being studied. Linear dichroism, as the name implies, involves measuring the difference in absorption of light linearly polarized parallel and perpendicular to an orientation axis. Linear dichroism provides information about the relative orientations of subunits of an interacting system. The material presented in this chapter is by no means comprehensive; the aim is to enable the user to collect reasonable quality data and to interpret it.

摘要

当一种药物与DNA结合时，其电子结构会受到扰动，同时也会扰动DNA的电子结构。电子光谱的这种变化可用于探究药物与DNA的相互作用。本章概述了如何利用圆二色光谱和线性二色光谱来提供有关药物 - DNA系统的信息。圆二色光谱涉及测量左旋和右旋圆偏振光吸收的差异。它对所研究分子的螺旋性具有独特的敏感性。顾名思义，线性二色性涉及测量平行和垂直于取向轴的线偏振光吸收的差异。线性二色性提供了关于相互作用系统亚基相对取向的信息。本章所呈现的内容绝非全面；目的是让使用者能够收集到质量合理的数据并对其进行解释。