Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China.
J Environ Sci (China). 2009;21(9):1169-74. doi: 10.1016/s1001-0742(08)62398-0.
A bench-scale cyclic activated sludge technology (CAST) was operated to study the biological phosphorus removal performance and a series of batch tests was carried out to demonstrate the accumulation of denitrifying polyphosphate-accumulating organisms (DNPAOs) in CAST system. Under all operating conditions, step-feed CAST with enough carbon sources in influent had the highest nitrogen and phosphorus removal efficiency as well as good sludge settling performance. The average removal rate of COD, NH4+-N, PO4(3-)-P and total nitrogen (TN) was 88.2%, 98.7%, 97.5% and 92.1%, respectively. The average sludge volume index (SVI) was 133 mL/g. The optimum anaerobic/aerobic/anoxic (AOA) conditions for the cultivation of DNPAOs could be achieved by alternating anoxic/oxic operational strategy, thus a significant denitrifying phosphorus removal occurred in step-feed CAST. The denitrification of NOx(-)-N completed quickly due to step-feed operation and enough carbon sources, which could enhance phosphorus release and further phosphorus uptake capability of the system. Batch tests also proved that polyphosphate-accumulating organisms (PAOs) in the step-feed process had strong denitrifying phosphorus removal capacity. Both nitrate and nitrite could be used as electron acceptors in denitrifying phosphorus removal. Low COD supply with step-feed operation strategy would favor DNPAOs accumulation.
采用实验室规模的循环活性污泥技术（CAST）研究了生物除磷性能，并进行了一系列批式试验以证明 CAST 系统中反硝化聚磷菌（DNPAOs）的积累。在所有操作条件下，进水具有足够碳源的分步进料 CAST 具有最高的氮磷去除效率和良好的污泥沉降性能。COD、NH4+-N、PO4(3-)-P 和总氮（TN）的平均去除率分别为 88.2%、98.7%、97.5%和 92.1%。平均污泥体积指数（SVI）为 133 mL/g。通过交替缺氧/好氧/缺氧（AOA）操作策略可以实现最佳的厌氧/好氧/缺氧（AOA）条件，从而在分步进料 CAST 中发生了显著的反硝化除磷。由于分步进料操作和足够的碳源，NOx(-)-N 的反硝化迅速完成，这可以增强系统的磷释放和进一步的磷吸收能力。批式试验还证明，分步进料过程中的聚磷菌（PAOs）具有很强的反硝化除磷能力。硝酸盐和亚硝酸盐都可以作为反硝化除磷的电子受体。分步进料操作策略下低 COD 供应有利于 DNPAOs 的积累。
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