富含多酚的苹果皮提取物对二十碳五烯酸和鱼油氧化的抗氧化保护作用。

Antioxidant protection of eicosapentaenoic acid and fish oil oxidation by polyphenolic-enriched apple skin extract.

机构信息

Department of Environmental Sciences, Nova Scotia Agricultural College, PO Box 550, Truro, Nova Scotia, Canada.

出版信息

J Agric Food Chem. 2010 Jan 27;58(2):1233-9. doi: 10.1021/jf903162k.

PMID:20000574

Abstract

Two apple skin extracts (ASE) prepared from "Northern Spy" cultivar were examined for their antioxidant properties to inhibit lipid oxidation in aqueous eicosapentaenoic acid (EPA) emulsions and bulk fish oil. The ASE were effective in reducing the oxidation induced by heat, UV light and peroxyl radical, when the extent of oxidation of the emulsions and bulk oil was measured by using the ferric thiocyanate test, the thiobarbituric acid reactive substances assay and Rancimat. On the basis of total phenolic concentration of extracts, removal of sugars and organic acids (ASE 2) from crude ethanol extract of apple skins (ASE 1) enhanced the antioxidant properties in both the emulsion and bulk fish oil systems. The average induction times of accelerated oxidation at 50 to 80 degrees C of fish oil incorporated with ASE 2 (400 microg/mL) was similar to that of alpha-tocopherol (400 microg/mL) and butylated hydroxytoluene (200 microg/mL).

摘要

两种从“北方间谍”品种苹果皮中提取的提取物（ASE），其抗氧化特性被检测以抑制水包二十碳五烯酸（EPA）乳液和块状鱼油中的脂质氧化。当使用铁氰化钾试验、硫代巴比妥酸反应物质测定法和 rancimat 法来测量乳液和块状油的氧化程度时，ASE 可以有效减少热、紫外线和过氧自由基诱导的氧化。基于提取物的总酚浓度，从苹果皮粗乙醇提取物中去除糖和有机酸（ASE 2）增强了乳液和块状鱼油体系中的抗氧化特性。添加 400μg/ml ASE 2 的鱼油在 50 至 80 摄氏度的加速氧化的平均诱导时间与α-生育酚（400μg/ml）和丁基化羟基甲苯（200μg/ml）相似。