Instituto de Biotecnología, Universidad Nacional de Colombia, Santafé de Bogotá DC, Colombia.
FEMS Microbiol Lett. 2010 Jan;302(2):93-8. doi: 10.1111/j.1574-6968.2009.01821.x. Epub 2009 Oct 15.
The use of Cry proteins from Bacillus thuringiensis are an important strategy for biological control. Recently it has been demonstrated that Cry hybrid proteins (by domain swapping) resulted in improved toxicities in comparison with parental proteins. Here, an SN1917 hybrid toxin was constructed and tested against Colombian pest insects Tecia solanivora (Lepidoptera: Gelechiidae), a severe potato pest, and Hypothenemus hampei (Coleoptera: Scolytidae), which attacks coffee crops. The SN1917 protoxin had a concentration causing 50% mortality (LC(50)) of 392 ng cm(-2), and SN1917 toxin showed an LC(50) of 201 ng cm(-2) against T. solanivora first instar larvae. The two parental toxins (Cry1B and Cry1I) used to make this new hybrid protein caused a mortality of 60% and 52%, respectively. Unfortunately, H. hampei first instar larvae proved to be resistant to the toxin. We conclude that SN1917 is an option for biological control and resistance management of T. solanivora. Implications for H. hampei are discussed.
苏云金芽孢杆菌的 Cry 蛋白的使用是生物防治的一个重要策略。最近已经证明，Cry 杂合蛋白（通过结构域交换）与亲本蛋白相比，导致了毒性的提高。在这里，构建了一种 SN1917 杂合毒素，并对哥伦比亚害虫番茄天蛾（鳞翅目：麦蛾科）和咖啡作物的主要害虫咖啡果小蠹（鞘翅目：小蠹科）进行了测试。SN1917 原毒素的半致死浓度（LC50）为 392ng/cm-2，SN1917 毒素对番茄天蛾初孵幼虫的 LC50 为 201ng/cm-2。用于制造这种新杂合蛋白的两种亲本毒素（Cry1B 和 Cry1I）分别导致 60%和 52%的死亡率。不幸的是，咖啡果小蠹的初孵幼虫对该毒素表现出抗性。我们得出结论，SN1917 是防治番茄天蛾和管理其抗性的一种选择。对咖啡果小蠹的影响也进行了讨论。
