Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Hum Pathol. 2010 Feb;41(2):271-80. doi: 10.1016/j.humpath.2009.07.022. Epub 2009 Dec 8.
CD81 is a tetraspanin cell surface protein that regulates CD19 expression in B lymphocytes and enables hepatitis C virus infection of human cells. Immunohistologic analysis in normal hematopoietic tissue showed strong staining for CD81 in normal germinal center B cells, a cell type in which its increased expression has not been previously recognized. High-dimensional flow cytometry analysis of normal hematopoietic tissue confirmed that among B- and T-cell subsets, germinal center B cells showed the highest level of CD81 expression. In more than 800 neoplastic tissue samples, its expression was also found in most non-Hodgkin lymphomas. Staining for CD81 was rarely seen in multiple myeloma, Hodgkin lymphoma, or myeloid leukemia. In hierarchical cluster analysis of diffuse large B-cell lymphoma, staining for CD81 was most similar to other germinal center B cell-associated markers, particularly LMO2. By flow cytometry, CD81 was expressed in diffuse large B-cell lymphoma cells independent of the presence or absence of CD10, another germinal center B-cell marker. The detection of CD81 in routine biopsy samples and its differential expression in lymphoma subtypes, particularly diffuse large B-cell lymphoma, warrant further study to assess CD81 expression and its role in the risk stratification of patients with diffuse large B-cell lymphoma.
CD81 是一种四跨膜蛋白，可调节 B 淋巴细胞中 CD19 的表达，并使丙型肝炎病毒能够感染人类细胞。在正常造血组织中的免疫组织化学分析显示，正常生发中心 B 细胞中 CD81 染色强烈，而之前并未发现该细胞类型中 CD81 表达增加。对正常造血组织的高维流式细胞术分析证实，在 B 细胞和 T 细胞亚群中，生发中心 B 细胞表达的 CD81 水平最高。在 800 多个肿瘤组织样本中，其表达也存在于大多数非霍奇金淋巴瘤中。在多发性骨髓瘤、霍奇金淋巴瘤或髓样白血病中很少见到 CD81 染色。在弥漫性大 B 细胞淋巴瘤的层次聚类分析中，CD81 的染色与其他生发中心 B 细胞相关标志物最为相似，尤其是 LMO2。通过流式细胞术，CD81 在弥漫性大 B 细胞淋巴瘤细胞中表达，与另一个生发中心 B 细胞标志物 CD10 的存在与否无关。在常规活检样本中检测到 CD81 及其在淋巴瘤亚型中的差异表达，特别是弥漫性大 B 细胞淋巴瘤，值得进一步研究以评估 CD81 表达及其在弥漫性大 B 细胞淋巴瘤患者分层风险中的作用。
