Center for Biologics Evaluation and Research, Food and Drug Administration, 8800 Rockville pike, Building 29A, 2B20, Bethesda, MD 20892, United States.
J Hazard Mater. 2010 Apr 15;176(1-3):414-7. doi: 10.1016/j.jhazmat.2009.11.045. Epub 2009 Nov 17.
Drop-to-drop solvent microextraction (DDSME) assisted with ultrasonication is applied for the determination of lead in one drop (30 microL) of blood sample by using graphite furnace atomic absorption spectrometry (GF-AAS). The optimum extraction efficiency of lead was observed for 10 min extraction time at pH 5.0 with 2 microL of organic solvent that containing 0.5 M of Cyanex-302. The optimized methodology exhibited good linearity in the range of 0.3-30.0 ng mL(-1) lead with relative standard deviations (RSD) from 2.5 to 4.4%. The method is found to be simple and rapid for the analysis of lead in micro amount of blood sample with the limit of detection (LOD) of 0.08 ng mL(-1). The application of the proposed method has been successfully tested for the determination of lead in blood samples. The results showed that under the optimized experimental conditions, the method showed good sensitivity and recovery %, as well as advantages such as linearity, simplicity, low cost and high feasibility.
采用超声辅助液滴溶剂微萃取(DDSME)，结合石墨炉原子吸收光谱法(GF-AAS)，对一滴(30 μL)血样中的铅进行测定。在 pH 值为 5.0 时，最优萃取条件为 10 min 萃取时间和 2 μL 含有 0.5 M 氰化-302 的有机溶剂，此时铅的萃取效率最高。优化后的方法在 0.3-30.0 ng mL(-1)的铅浓度范围内呈现良好的线性关系，相对标准偏差(RSD)为 2.5-4.4%。该方法简单快速，可用于分析微量血样中的铅，检出限(LOD)为 0.08 ng mL(-1)。该方法已成功应用于血液样品中铅的测定。结果表明，在优化的实验条件下，该方法具有良好的灵敏度和回收率，以及线性、简单、低成本和高可行性等优点。
