Institut für Mikrobiologie, Ernst-Moritz-Arndt-Universität, F.-L.-Jahn-Strasse 15, D-17487 Greifswald, Germany.
Int J Med Microbiol. 2010 Feb;300(2-3):76-87. doi: 10.1016/j.ijmm.2009.10.006. Epub 2009 Dec 11.
Staphylococcus aureus is a human pathogen that has advanced to a main problem in hospital settings since effective treatment options for infections caused by this pathogen are limited. Thus, new strategies to prevent and treat S. aureus infections and rapid diagnostic tools are urgently needed. The course of an S. aureus infection largely depends on successful adaptation to the host environment and a very complex and poorly understood interplay of bacterial virulence factors with each other and with host components. Over the last years, genome sequences of different S. aureus strains have been published permitting a high-throughput proteomic analysis of this pathogen. This review summarizes the impact of 2D gel- and mass spectrometry-based proteomic approaches on a more comprehensive understanding of S. aureus pathophysiology and virulence. We show that only a combination of both techniques allows a proteomic view which adequately considers all subproteomic fractions of a bacterium, i.e. cytosolic, membrane, cell surface-associated, and extracellular proteins. By this means, the majority of proteins expressed in S. aureus can be identified and even quantified. In addition, posttranslational processes such as protein secretion, modification, processing, damages, and degradation can be adequately studied.
金黄色葡萄球菌是一种人类病原体，由于治疗这种病原体引起的感染的有效治疗方法有限，因此已成为医院环境中的主要问题。因此，迫切需要新的策略来预防和治疗金黄色葡萄球菌感染和快速诊断工具。金黄色葡萄球菌感染的过程在很大程度上取决于其对宿主环境的成功适应，以及细菌毒力因子之间以及与宿主成分之间非常复杂且了解甚少的相互作用。在过去的几年中，不同金黄色葡萄球菌菌株的基因组序列已经发表，这使得对该病原体进行高通量蛋白质组学分析成为可能。这篇综述总结了基于 2D 凝胶和质谱的蛋白质组学方法在更全面地了解金黄色葡萄球菌病理生理学和毒力方面的影响。我们表明，只有将这两种技术结合使用，才能对细菌的所有亚蛋白质组部分（即细胞质、膜、细胞表面相关和细胞外蛋白质）进行全面的蛋白质组学观察。通过这种方法，可以鉴定和定量表达的大多数金黄色葡萄球菌蛋白。此外，可以充分研究蛋白质分泌、修饰、加工、损伤和降解等翻译后过程。
