Wei Z, Fan L, Xiangming C
Department of General Surgery, The First Hospital, Medical College Xi'an Jiao Tong University, Xi'an, Shan'xi Province, China.
Transplant Proc. 2009 Dec;41(10):4120-2. doi: 10.1016/j.transproceed.2009.09.054.
The present study was designed to examine whether ischemic preconditioning could play a protective role on cold ischemia and reperfusion injury associated with intestinal transplantation in rats.
The 48 male Sprague Dawley (SD) rats were randomly assigned to 2 groups. Ischemic preconditioning was performed in experimental but not control rats by preserving the self donor small bowel in Ringer lactate solution at 4 degrees C for 3 hours (n = 6), 6 hours (n = 6), 12 hours (n = 6), or 18 hours (n = 6). One hour reperfusion was performed for every rat after orthotopic transplantation of donor small bowel. Small bowel samples were obtained for histological examination and immunohistochemistry analysis of nuclear factor kappaB (NF-kappaB) expression.
The small intestinal villus was arranged more regularly in experimental compared with control rats. Ischemia preconditioning also decreased edema in the muscule layer and increased Chiu score in experimental rats. Immunohistochemistry analysis revealed that ischemic preconditioning down-regulated the expression of NF-kappaB in the epithelia of experimental rats.
Ischemic preconditioning improved intestinal transplantation in rats from cold ischemia and reperfusion injury by down-regulating the expression of NF-kappaB.
本研究旨在探讨缺血预处理是否能对大鼠小肠移植相关的冷缺血和再灌注损伤起到保护作用。
将48只雄性Sprague Dawley（SD）大鼠随机分为2组。对实验组大鼠进行缺血预处理，即在4℃的乳酸林格液中保存自体供体小肠3小时（n = 6）、6小时（n = 6）、12小时（n = 6）或18小时（n = 6），而对照组大鼠不进行预处理。在供体小肠原位移植后，对每只大鼠进行1小时的再灌注。获取小肠样本进行组织学检查和核因子κB（NF-κB）表达的免疫组织化学分析。
与对照组大鼠相比，实验组大鼠小肠绒毛排列更规则。缺血预处理还减轻了实验组大鼠肌层的水肿并提高了Chiu评分。免疫组织化学分析显示，缺血预处理下调了实验组大鼠上皮细胞中NF-κB的表达。
缺血预处理通过下调NF-κB的表达改善了大鼠小肠移植的冷缺血和再灌注损伤。
