Key B, Akeson R A
Division of Basic Research, Children's Hospital Research Foundation, Cincinnati, Ohio 45229.
Neuron. 1991 Mar;6(3):381-96. doi: 10.1016/0896-6273(91)90247-w.
The olfactory neuroepithelium, which contains the primary sensory olfactory neurons, continually undergoes neurogenesis and axonal outgrowth throughout life. We describe here several new olfactory system-specific glycoforms of the neural cell adhesion molecule N-CAM in the frog, R. catesbeiana. Using immunochemical methods for in situ localization, we show that the lectin dolichos biflorus agglutinin (DBA) and two monoclonal antibodies, 9OE and 3A6, detect three unique N-CAM forms present on primary sensory olfactory axons. In addition, DBA and monoclonal antibody 9OE recognize glycoconjugates and/or N-CAM glycoforms expressed specifically in discrete central olfactory pathways and regions in frog brain. This is a novel example of unique adhesion molecule forms present in a chain of two neurons within a vertebrate neural pathway. Together these glycoconjugates and N-CAM glycoforms may participate in cellular interactions associated with olfactory system pathway formation and renewal.
嗅觉神经上皮包含初级感觉嗅觉神经元，在整个生命过程中持续进行神经发生和轴突生长。我们在此描述了牛蛙（R. catesbeiana）中神经细胞黏附分子N-CAM的几种新的嗅觉系统特异性糖型。通过免疫化学原位定位方法，我们发现凝集素双花扁豆凝集素（DBA）和两种单克隆抗体9OE和3A6可检测到初级感觉嗅觉轴突上存在的三种独特的N-CAM形式。此外，DBA和单克隆抗体9OE识别在青蛙大脑中离散的中枢嗅觉通路和区域中特异性表达的糖缀合物和/或N-CAM糖型。这是脊椎动物神经通路中两个神经元链中存在独特黏附分子形式的一个新例子。这些糖缀合物和N-CAM糖型共同可能参与与嗅觉系统通路形成和更新相关的细胞相互作用。
