Wegener Christian, Neupert Susanne, Predel Reinhard
Emmy Noether Neuropeptide Group, Animal Physiology, Philipps-University, Marburg, Germany.
Methods Mol Biol. 2010;615:117-27. doi: 10.1007/978-1-60761-535-4_9.
Direct MALDI-TOF mass spectrometric peptide profiling is increasingly used to analyze the peptide complement in the nervous system of a variety of invertebrate animals, from leech to Aplysia and many arthropod species, especially insects and crustaceans. Proper sample preparation is often the most crucial step to obtain the necessary data. Here, we describe protocols for the use of MALDI-TOF mass spectrometry to directly analyze the peptidome of neuroendocrine tissues of insects, particularly Drosophila melanogaster, by MALDI-TOF MS.
直接基质辅助激光解吸电离飞行时间质谱肽谱分析越来越多地用于分析从水蛭到海兔以及许多节肢动物物种，特别是昆虫和甲壳类动物等各种无脊椎动物神经系统中的肽组。合适的样品制备通常是获得必要数据的最关键步骤。在此，我们描述了使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）直接分析昆虫，特别是黑腹果蝇神经内分泌组织肽组的实验方案。
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