用于生成转基因动物（包括绝缘子）的表达盒设计。
Design of expression cassettes for the generation of transgenic animals (including insulators).
作者信息
Houdebine Louis-Marie
机构信息
Département de Physiologie Animale, Institut National de la Recherche Agronomique, Nouzilly, France.
出版信息
Methods Mol Biol. 2010;597:55-69. doi: 10.1007/978-1-60327-389-3_4.
The use of transgenesis is relatively rare in rats, and this is because of the relative difficulty in adding foreign genes by the conventional methods. Gene knock out and knock in by the conventional techniques of homologous recombination remain difficult in rats. This situation would be less crucial if the gene constructs were more reliable for the expression of foreign genes. The present chapter describes the state of the art in vector design for various genetic modifications in rats.
转基因技术在大鼠中的应用相对较少，这是因为通过传统方法添加外源基因相对困难。利用同源重组的传统技术进行基因敲除和敲入在大鼠中仍然很困难。如果基因构建体对外源基因的表达更可靠，这种情况就不那么关键了。本章描述了大鼠各种基因修饰载体设计的最新技术水平。
Zotero 插件