Cozzi Jean, Wang Eryao, Jacquet Christelle, Fraichard Alexandre, Cherifi Yacine, Zhou Qi
genOway, SA, Lyon, France.
Methods Mol Biol. 2010;597:137-50. doi: 10.1007/978-1-60327-389-3_10.
Somatic cell nuclear transfer (SCNT) is a powerful tool for the investigation of the mechanisms of nuclear remodeling. In addition, SCNT may offer the possibility of introducing targeted mutations by homologous recombination in species for which ES cell technology is not available. The rat specific features of the oocyte have long impeded the development of SCNT. We detail here the procedures developed and optimized during the last several years for the optimization of rat cloning.
体细胞核移植（SCNT）是研究核重塑机制的有力工具。此外，对于无法应用胚胎干细胞技术的物种，SCNT可能提供通过同源重组引入靶向突变的可能性。大鼠卵母细胞的特殊特征长期以来阻碍了SCNT的发展。我们在此详细介绍过去几年中为优化大鼠克隆而开发和优化的程序。
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