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[大鼠氧化应激与β细胞脂性凋亡的关系]

[Relationship between oxidative stress and beta cell lipoapoptosis in rats].

作者信息

Lou Da-Jun, Li Hong-Liang, Yang Wen-Ying, Xiao Jian-Zhong, Du Rui-Qin, Wang Bing, Bai Xiu-Ping, Pan Lin

机构信息

Department of Endocrinology, China-Japan Friendship Hospital, Beijing 100029, China.

出版信息

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2009 Nov;38(6):620-5. doi: 10.3785/j.issn.1008-9292.2009.06.011.

Abstract

OBJECTIVE

To investigate the effect of beta cell lipoapoptosis after long term high-fat feeding in rats, and to investigate the relationship between oxidative stress, gene expression and beta cell lipoapoptosis.

METHODS

Forty-one SD male rats were randomly divided into 2 groups: high-fat diet group (HF group) and control group (NC group). At the end of 28 weeks, the levels of malondialdehyde (MDA) and glutamylcysteinylglycine (GSH) in plasma and pancreatic tissue,the early-phase insulin secretion in beta cells, the beta cell apoptosis (TUNEL technology) and the uncoupling protein 2 (UCP2) gene expression in islets were measured.

RESULT

The concentrations of MDA both in plasma and pancreatic tissue were higher in HF group than those in NC group.In contrast, The contents of GSH both in plasma and pancreatic tissue were lower in HF group. Insulin secretion response to glucose load was significantly decreased in HF group (3.0 fold Compared with 5.7 fold, P<0.01). Blood glucose levels at 3 min, 5 min and 10 min during IVGTT were significantly higher in HF group than those in NC group (P<0.05). The frequency of beta cell apoptosis was increased by 40.0% in HF group (P<0.01). The gene expression of UCP2 in islets was increased by 22.4% in HF group (P<0.01).

CONCLUSION

The frequency of beta cell apoptosis in high-fat feeding rats is affected by oxidative stress, which results in increasing UCP2 gene expression.

摘要

目的

研究长期高脂喂养对大鼠β细胞脂肪性凋亡的影响,探讨氧化应激、基因表达与β细胞脂肪性凋亡之间的关系。

方法

将41只SD雄性大鼠随机分为2组:高脂饮食组(HF组)和对照组(NC组)。28周结束时,检测血浆和胰腺组织中丙二醛(MDA)和谷胱甘肽(GSH)水平、β细胞早期胰岛素分泌、β细胞凋亡(TUNEL技术)以及胰岛中解偶联蛋白2(UCP2)基因表达。

结果

HF组血浆和胰腺组织中MDA浓度均高于NC组。相比之下,HF组血浆和胰腺组织中GSH含量均较低。HF组对葡萄糖负荷的胰岛素分泌反应显著降低(分别为3.0倍和5.7倍,P<0.01)。静脉葡萄糖耐量试验期间3分钟、5分钟和10分钟时HF组血糖水平显著高于NC组(P<0.05)。HF组β细胞凋亡频率增加40.0%(P<0.01)。HF组胰岛中UCP2基因表达增加22.4%(P<0.01)。

结论

高脂喂养大鼠β细胞凋亡频率受氧化应激影响,导致UCP2基因表达增加。

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