Toll 样受体 4 通过 Cox-2 和 PGE2 参与胃黏膜保护。

Toll-like receptor 4 participates in gastric mucosal protection through Cox-2 and PGE2.

机构信息

Division of Gastroenterology, West China Hospital of Sichuan University, GuoXue Street 37, Chengdu 610041, Sichuan, China.

出版信息

Dig Liver Dis. 2010 Jul;42(7):472-6. doi: 10.1016/j.dld.2009.10.007. Epub 2009 Dec 16.

DOI:10.1016/j.dld.2009.10.007

PMID:20018573

Abstract

AIM

To elucidate the role of Toll-like receptors (TLRs) in gastric cytoprotection after ethanol injury.

METHODS

C57BL/6J, C3H/HeOuJ and C3H/HeJ mice were used. All mice were killed 4h after ethanol administration. TLR4, cyclooxygenase-2 (Cox-2) and prostaglandin E(2) (PGE(2)) expression were measured by immunohistochemistry, western blotting and enzyme-linked immunosorbent assay (ELISA) separately.

RESULTS

The expression of TLR4 increased in C57BL/6J mice stomach 4h after ethanol injury. The cells expressing TLR4 included Cox-2 expressing cells and macrophages. The injury in C3H/HeJ mice was more severe than in C3H/HeOuJ mice 4h after ethanol injury. The expression of Cox-2 and PGE(2) only increased in C3H/HeOuJ mice. The number of macrophages and the expression of macrophage-inflammatory protein-2 (MIP-2) also increased only in C3H/HeOuJ mice.

CONCLUSION

TLR4 signal is activated in mice stomach 4h after ethanol injury. The protective effects of TLR4 signalling are mediated through the induction of Cox-2 expression and the production of PGE(2).

摘要

目的

阐明 Toll 样受体（TLRs）在乙醇损伤后胃细胞保护中的作用。

方法

使用 C57BL/6J、C3H/HeOuJ 和 C3H/HeJ 小鼠。所有小鼠在乙醇给药后 4 小时处死。分别通过免疫组织化学、western blot 和酶联免疫吸附试验（ELISA）测定 TLR4、环氧化酶-2（Cox-2）和前列腺素 E（PGE（2））的表达。

结果

乙醇损伤后 4 小时，C57BL/6J 小鼠胃中 TLR4 的表达增加。表达 TLR4 的细胞包括 Cox-2 表达细胞和巨噬细胞。与 C3H/HeOuJ 小鼠相比，乙醇损伤后 4 小时，C3H/HeJ 小鼠的损伤更为严重。仅在 C3H/HeOuJ 小鼠中，Cox-2 和 PGE（2）的表达增加。巨噬细胞数量和巨噬细胞炎性蛋白-2（MIP-2）的表达也仅在 C3H/HeOuJ 小鼠中增加。