Key Laboratory of Biomedical Polymers, Ministry of Education, Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Macromol Biosci. 2010 Apr 8;10(4):404-14. doi: 10.1002/mabi.200900300.
Dendritic poly(amidoamine)s (PAMAM)s were introduced into the side chains of disulfide-containing poly(amidoamine)s via repetitive Michael addition and amidation. The bioreducible poly(amidoamine)s grafted with dendritic polyamidoamines showed high buffer capacity, low cytotoxicity and strong DNA binding ability at low N/P ratio. They were able to condense DNA into small sized polycation/DNA complexes, which degraded and released the incorporated DNA under reductive conditions. In comparison to the original disulfide-containing poly(amidoamine) with aminoethyl side chain, the grafting of the bioreducible poly(amidoamine) with dendrimer greatly improved the transfection efficiencies of 293T and HeLa cells with foreign DNA at various N/P ratios. The structure-gene delivery property relations of dendrimer-grafted polycations will provide valuable insight into the design of highly efficient and less toxic polycationic gene carriers.
树枝状聚酰胺-胺（PAMAM）通过反复的迈克尔加成和酰胺化反应被引入含二硫键的聚酰胺-胺的侧链中。接枝有树枝状聚酰胺胺的还原性聚酰胺-胺具有较高的缓冲能力、低细胞毒性和在低 N/P 比下与 DNA 强结合能力。它们能够将 DNA 凝聚成小尺寸的聚阳离子/DNA 复合物，在还原条件下这些复合物能够降解并释放出所包含的 DNA。与具有氨乙基侧链的原始含二硫键的聚酰胺-胺相比，接枝有生物还原性聚酰胺-胺的树枝状聚合物大大提高了 293T 和 HeLa 细胞在不同 N/P 比下对外源 DNA 的转染效率。树枝状聚合物接枝聚阳离子的结构-基因传递性质关系将为设计高效、低毒的聚阳离子基因载体提供有价值的见解。
