U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health; Division of Applied Research and Technology, Taft Laboratory, 4676 Columbia Parkway, Cincinnati, Ohio, 45226, USA.
Toxicol Mech Methods. 2005;15(5):367-73. doi: 10.1080/153765291009976.
A test procedure for the determination of (2-methoxyethoxy)acetic acid (MEAA) was adapted and applied to urine samples from jet fuel (JP-8)-exposed mice using capillary gas chromatography with a mass selective detector (MSD). MEAA is a metabolite and proposed biomarker for exposure to 2-(2-methoxyethoxy)ethanol, a glycol ether component in the formulation of JP-8. The collected urine samples were spiked with deuterated butoxyacetic acid internal standard, and extracted with ethyl acetate, and esterified with ethanol and sulfuric acid, and the esters of the glycol ethers were extracted with methylene chloride. The chromatographic conditions used easily separate the MEAA ethyl ester from interferences within mouse urine. The application of this procedure to urine samples collected from mice demonstrated that MEAA was detectable after oral (2000 mg/kg) or dermal (50 mu L) exposure for 7 days to JP-8 at levels as high as 8.5 or 6.5 mu g/mL, respectively. This pilot demonstration indicated that total urinary MEAA was a viable biomarker for the two routes of JP-8 exposure in laboratory mice.
适应并采用一种（2-甲氧基乙氧基）乙酸（MEAA）的测试程序，使用带有质量选择性检测器（MSD）的毛细管气相色谱法，对喷气燃料（JP-8）暴露的小鼠尿液样本进行分析。MEAA 是 2-（2-甲氧基乙氧基）乙醇的代谢物和拟生物标志物，2-（2-甲氧基乙氧基）乙醇是 JP-8 配方中的乙二醇醚成分。收集的尿液样本用氘代丁氧基乙酸内标物进行处理，用乙酸乙酯提取，用乙醇和硫酸酯化，乙二醇醚的酯用二氯甲烷提取。所使用的色谱条件可轻松将 MEAA 乙酯与小鼠尿液中的内部分离。该程序应用于从小鼠尿液中收集的样本，结果表明，口服（2000mg/kg）或经皮（50μL）暴露于 JP-8 7 天后，MEAA 可高达 8.5 或 6.5μg/ml 的水平检测到，分别。该初步研究表明，尿液中总 MEAA 是实验室小鼠两种 JP-8 暴露途径的可行生物标志物。
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