Departments of Pathology, Kangdong Sacred-Heart Hospital, Hallym University Medical College, Seoul.
Pathol Int. 2009 Dec;59(12):851-6. doi: 10.1111/j.1440-1827.2009.02465.x.
The aim of the present study was to compare the efficiency of tissue microarray (TMA) results using immunohistochemistry markers applied to a variety of core sizes and full sections of gastrointestinal stromal tumors (GIST) using performance measures such as core loss rate and concordance rate. Six primary antibody markers (c-Kit, CD34, smooth muscle actin (SMA), S-100, p53, and Ki-67) were used with the TMA technique to analyze 0.6 mm, 2 mm, and 3 mm punch cores of GIST samples from 67 patients. No statistical association was found between core size and loss rate (P= 0.512). TMA results for the 0.6 mm, 2 mm, and 3 mm core showed that all core sizes could statistically significantly reflect full sections with regard to c-Kit, SMA, and S-100 antibodies, but that the 3 mm core section was the most representative except for CD34. With regard to p53 and Ki-67 staining, the 0.6 mm core section was not representative, but the 2 mm and 3 mm core sections could statistically significantly represent full section results. Among them, the 3 mm core section was more accurate than the 2 mm core section. Use of a single 3 mm core size in TMA is suitable for evaluating large numbers of protein and nuclear stains with regard to immunohistochemistry for GIST.
本研究旨在比较使用组织微阵列（TMA）技术，应用不同大小的核心和胃肠道间质瘤（GIST）的全切片，使用核心丢失率和一致性率等性能指标，分析免疫组织化学标记物的效率。使用 TMA 技术分析了 67 名患者的 0.6mm、2mm 和 3mm 打孔核心的 GIST 样本，共使用了 6 种主要的抗体标记物（c-Kit、CD34、平滑肌肌动蛋白（SMA）、S-100、p53 和 Ki-67）。未发现核心大小与丢失率之间存在统计学关联（P=0.512）。0.6mm、2mm 和 3mm 核心的 TMA 结果表明，所有核心大小在 c-Kit、SMA 和 S-100 抗体方面都可以统计学显著反映全切片，而 3mm 核心切片是最具代表性的，除了 CD34。关于 p53 和 Ki-67 染色，0.6mm 核心切片不具有代表性，但 2mm 和 3mm 核心切片可以统计学显著代表全切片结果。其中，3mm 核心切片比 2mm 核心切片更准确。在 TMA 中使用单个 3mm 核心大小适用于评估大量 GIST 的免疫组织化学的蛋白质和核染色。
