Liu Li, Ding Yan-qing
Department of Health Management, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Zhonghua Bing Li Xue Za Zhi. 2009 Aug;38(8):529-31.
To investigate the effect of protein kinase CK2alpha expression on the proliferation and invasion of human nasopharyngeal carcinoma and related mechanism.
RNA interference (RNAi) was used to down-regulate the protein kinase CK2alpha expression in 5-8F cells. The biological behavior of treated cells were analyzed by MTT and in-vitro invasion assays. Cell proliferation cycle was examined by flow cytometry and the phosphorylation status of Akt protein was examined by Western blotting analysis.
CK2alpha protein was successfully silenced by siRNA. CK2alpha knockdown significantly decreased the proliferative and invasive abilities of 5-8F cells. Flow cytometry analysis showed that the percentage of cells increased in G(0)/G(1) phase but decreased in S phase. Moreover, the expression of phosphorylated Akt was down-regulated by CK2alpha silencing.
Protein kinase CK2alpha plays an important role in the proliferation and invasion of nasopharyngeal carcinoma, and may provide a potential therapeutic target against nasopharyngeal cancer.
探讨蛋白激酶CK2α表达对人鼻咽癌增殖和侵袭的影响及其相关机制。
采用RNA干扰（RNAi）技术下调5-8F细胞中蛋白激酶CK2α的表达。通过MTT法和体外侵袭实验分析处理后细胞的生物学行为。采用流式细胞术检测细胞增殖周期，并用蛋白质印迹分析检测Akt蛋白的磷酸化状态。
siRNA成功沉默了CK2α蛋白。CK2α基因敲低显著降低了5-8F细胞的增殖和侵袭能力。流式细胞术分析显示，G(0)/G(1)期细胞百分比增加，S期细胞百分比降低。此外，CK2α沉默下调了磷酸化Akt的表达。
蛋白激酶CK2α在鼻咽癌的增殖和侵袭中起重要作用，可能为鼻咽癌提供潜在的治疗靶点。
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