Laboratoire de Physique de la Matière Vivante, IPSB, BSP, Ecole Polytechnique Fédérale de Lausanne, CH1015 Lausanne, Switzerland.
Ultramicroscopy. 2010 Feb;110(3):211-5. doi: 10.1016/j.ultramic.2009.11.011. Epub 2009 Nov 26.
We report the first use of polymethylmethacrylate (PMMA) optical fiber-made probes for scanning near-field optical microscopy (SNOM). The sharp tips were prepared by chemical etching of the fibers in ethyl acetate, and the probes were prepared by proper gluing of sharpened fibers onto the tuning fork in the conditions of the double resonance (working frequency of a tuning fork coincides with the resonance frequency of dithering of the free-standing part of the fiber) reported earlier for the case of glass fibers. Quality factors of the probes in the range 2000-6000 were obtained, which enables the realization of an excellent topographical resolution including state-of-art imaging of single DNA molecules. Near-field optical performance of the microscope is illustrated by the Photon Scanning Tunneling Microscope images of fluorescent beads with a diameter of 100nm. The preparation of these plastic fiber probes proved to be easy, needs no hazardous material and/or procedures, and typical lifetime of a probe essentially exceeds that characteristic for the glass fiber probe.
我们报告了首次使用聚甲基丙烯酸甲酯(PMMA)光纤制作的探针进行扫描近场光学显微镜(SNOM)。通过在乙酸乙酯中对光纤进行化学蚀刻来制备锋利的尖端,并且通过在早先报道的玻璃光纤情况下的双共振(调谐叉的工作频率与光纤自由摆动部分的共振频率相吻合)条件下,将经过锐化的光纤适当粘接到调谐叉上来制备探针。在 2000-6000 的范围内获得了探针的品质因数,这使得能够实现包括单 DNA 分子的最新成像技术在内的优异的形貌分辨率。通过直径为 100nm 的荧光珠的光子扫描隧道显微镜图像说明了显微镜的近场光学性能。这些塑料光纤探针的制备证明是容易的,不需要危险的材料和/或程序,并且探针的典型寿命实质上超过了玻璃光纤探针的特征寿命。