Department of Chemistry, University of Sistan & Baluchestan, Zahedan, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2010 Feb;75(2):598-603. doi: 10.1016/j.saa.2009.11.024. Epub 2009 Dec 22.
The interaction of [Nd(bpy)(2)Cl(3)xOH(2)], where bipy is 2,2'-bipyridine, with DNA has been studied by absorption, emission, and viscosity measurements. [Nd(bpy)(2)Cl(3)xOH(2)] showed absorption decreasing in charge transfer band with increasing of DNA. The binding constant, K(b) has been determined by absorption measurement and found to be (1.5+/-0.1)x10(5)M(-1). The fluorescent of [Nd(bpy)(2)Cl(3)xOH(2)] has been investigated in detail. The interaction was also studied by fluorescence quenching technique. The results of fluorescence titration revealed that DNA had the strong ability to quenching the intrinsic fluorescence of Nd(III) complex at 327 nm. The binding site number n, apparent binding constant K(b) and the Stern-Volmer quenching constant K(SV) have been determined. Thermodynamic parameters have been calculated according to relevant fluorescent data and Van't Hoff equation. Characterization of bonding mode has been studied. The results suggested that the major interaction mode between [Nd(bpy)(2)Cl(3)xOH(2)] and DNA was groove binding.
[Nd(bpy)(2)Cl(3)xOH(2)]（bpy 是 2,2'-联吡啶）与 DNA 的相互作用通过吸收、发射和粘度测量进行了研究。随着 DNA 浓度的增加，[Nd(bpy)(2)Cl(3)xOH(2)]的荷移带吸收逐渐降低。通过吸收测量确定了结合常数 K(b)，并发现其为 (1.5+/-0.1)x10(5)M(-1)。详细研究了 [Nd(bpy)(2)Cl(3)xOH(2)]的荧光。还通过荧光猝灭技术研究了相互作用。荧光滴定的结果表明，DNA 具有很强的能力，可以猝灭 Nd(III)配合物在 327nm 处的本征荧光。确定了结合位点数 n、表观结合常数 K(b)和 Stern-Volmer 猝灭常数 K(SV)。根据相关荧光数据和范特霍夫方程计算了热力学参数。研究了键合模式的特征。结果表明，[Nd(bpy)(2)Cl(3)xOH(2)]与 DNA 之间的主要相互作用模式是沟槽结合。
