Schaerli Yolanda, Hollfelder Florian
Department of Biochemistry, University of Cambridge, Cambridge, UK.
Mol Biosyst. 2009 Dec;5(12):1392-404. doi: 10.1039/b907578j. Epub 2009 Oct 12.
The comprehensive characterisation of complex parameter space in '-omics' technologies requires high-throughput systems. In vitro compartmentalisation of reactions in water-in-oil droplets combines the necessary ability to carry out large numbers of experiments under controlled conditions with quantitative readout, and has recently advanced towards automation by generating droplets in microfluidic devices. Some approaches based on these principles are already familiar (e.g. emulsion PCR for sequencing), others, including directed evolution or cell-based assays, are in advanced stages of development--and proof-of-principle experiments are appearing for a whole range of applications in diagnostics, cellomics, proteomics, drug discovery and systems and synthetic biology. This review describes the current state-of-the-art, notes salient features of successful experiments and extrapolates in the direction of more highly integrated systems.
“组学”技术中复杂参数空间的全面表征需要高通量系统。油包水液滴中的反应体外分区，将在受控条件下进行大量实验的必要能力与定量读数相结合，并且最近通过在微流控装置中生成液滴而朝着自动化发展。一些基于这些原理的方法已经为人所熟知（例如用于测序的乳液PCR），其他方法，包括定向进化或基于细胞的分析，正处于开发的高级阶段——并且原理验证实验正在诊断、细胞组学、蛋白质组学、药物发现以及系统和合成生物学等一系列应用中出现。本综述描述了当前的技术水平，指出了成功实验的显著特征，并朝着更高集成度系统的方向进行了推断。
