Department of Chemistry, Seoul National University, Seoul, 151-742, South Korea.
Anal Bioanal Chem. 2010 May;397(2):557-62. doi: 10.1007/s00216-009-3365-9. Epub 2009 Dec 22.
A novel method to deposit a highly surface-enhanced Raman scattering (SERS) active silver film onto the inside surface of a glass capillary is developed. Firstly, Ag sol was synthesized by the reaction of AgNO(3) with poly-(ethylenimine) (PEI), and then toluene and benzenethiol (BT) were added into the sol. The mixture was flowed through the glass capillary to obtain the SERS-active Ag film-coated glass capillary. The SERS activity of the Ag-coated capillary was dependent on the amount of PEI and BT used. In addition, BT could be easily desorbed from the Ag surface by treating it with a borohydride solution, maintaining the initial SERS activity. The SERS enhancement factor at 632.8-nm excitation was estimated to be on the order of 10(6). The detection limits of adenine and dipicolinic acid were then as low as 1.0 x 10(-8) and 1.0 x 10(-7) M, respectively, based on an S/N ratio of 3. This clearly suggests that the Ag-coated capillary is an invaluable device for the analysis of effluent chemicals by SERS.
一种将高度表面增强拉曼散射（SERS）活性的银膜沉积到玻璃毛细管内表面的新方法被开发出来。首先，通过 AgNO3 与聚（乙二胺）（PEI）的反应合成 Ag 溶胶，然后将甲苯和苯硫酚（BT）加入到溶胶中。将混合物流经玻璃毛细管，获得具有 SERS 活性的 Ag 膜涂覆的玻璃毛细管。Ag 涂层毛细管的 SERS 活性取决于所用 PEI 和 BT 的量。此外，通过用硼氢化物溶液处理，可以将 BT 从 Ag 表面轻易解吸，从而保持初始 SERS 活性。在 632.8nm 激发下的 SERS 增强因子估计在 10^6 量级。然后，基于信噪比为 3，腺嘌呤和二吡啶甲酸的检测限分别低至 1.0 x 10^(-8) 和 1.0 x 10^(-7) M。这清楚地表明，Ag 涂层毛细管是通过 SERS 分析流出化学物质的宝贵装置。
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