Ohio State University, Department of Entomology, 318 W. 12th Ave., Columbus, OH, USA.
J Insect Physiol. 2010 Jun;56(6):603-9. doi: 10.1016/j.jinsphys.2009.12.007. Epub 2009 Dec 30.
Subtractive suppressive hybridization (SSH) was used to characterize the diapause transcriptome of the flesh fly Sarcophaga crassipalpis. Through these efforts, we isolated 97 unique clones which were used as probes in northern hybridization to assess their expression during diapause. Of these, 17 were confirmed to be diapause upregulated and 1 was diapause downregulated, while 12 were shown to be unaffected by diapause in this species. The diapause upregulated genes fall into several broad categories including heat shock proteins, heavy metal responsive genes, neuropeptides, structural genes, regulatory elements, and several genes of unknown function. In combination with other large-scale analyses of gene expression during diapause, this study assists in the characterization of the S. crassipalpis diapause transcriptome, and begins to identify common elements involved in diapause across diverse taxa.
差减抑制杂交（SSH）被用于描述肉蝇 Sarcophaga crassipalpis 的滞育转录组。通过这些努力，我们分离了 97 个独特的克隆，并用它们作为探针进行 northern 杂交，以评估它们在滞育期间的表达情况。其中，17 个被证实是滞育上调的，1 个是滞育下调的，而 12 个在该物种中不受滞育影响。滞育上调的基因属于几个广泛的类别，包括热休克蛋白、重金属反应基因、神经肽、结构基因、调节元件和几个功能未知的基因。结合其他对滞育期间基因表达的大规模分析，本研究有助于对 S. crassipalpis 滞育转录组进行特征描述，并开始鉴定不同分类群中参与滞育的共同元素。
