谷胱甘肽 S-转移酶 M1 和 T1 多态性与韩国健康吸烟者氧化损伤易感性的关系。

Glutathione s-transferase m1 and t1 polymorphisms and susceptibility to oxidative damage in healthy korean smokers.

机构信息

Department of Food and Nutrition, Hanyang University, Seoul, Korea.

出版信息

Ann Nutr Metab. 2010;56(1):52-8. doi: 10.1159/000268018. Epub 2009 Dec 18.

DOI:10.1159/000268018

PMID:20029178

Abstract

AIMS

The relationship between glutathione S-transferase (GST) polymorphisms and susceptibility to smoking-induced oxidative damage was investigated in healthy Korean smokers.

METHODS

Forty-nine healthy male smokers (20-59 years) participated in the study. GSTT1 and GSTM1 genotypes were determined, and lymphocyte DNA damage was assessed by the Comet assay.

RESULTS

Out of the 49 smokers, 20 individuals were GSTT1 positive and 29 were GSTT1 null. For GSTM1, 45 smokers were GSTM1 positive and 4 were GSTM1 null. HDL cholesterol levels (p = 0.011) and the atherogenic index of plasma (p = 0.01) were significantly reduced, and concentrations of conjugated dienes were increased (p = 0.01) in the GSTT1-null individuals but not in the GSTT1-positive individuals. The GSTT1-null individuals were more susceptible to DNA damage than the GSTT1-positive individuals. For all parameters, the GSTM1 genotypes were excluded from statistical analyses due to the small number of subjects with the GSTM1-null type.

CONCLUSIONS

Our findings suggest that subjects with the GSTT1-null genotype were more susceptible to oxidative damage than the GSTT1-positive subjects. Therefore, the GSTT1-null genotype may confer an increased risk of smoking-related cardiovascular disease.

摘要

目的

本研究旨在探讨谷胱甘肽 S-转移酶（GST）多态性与吸烟诱导的氧化损伤易感性之间的关系。

方法

49 名健康的韩国男性吸烟者（20-59 岁）参与了本研究。通过彗星试验评估 GSTT1 和 GSTM1 基因型，并检测淋巴细胞 DNA 损伤。

结果

在 49 名吸烟者中，有 20 名个体为 GSTT1 阳性，29 名个体为 GSTT1 缺失。对于 GSTM1，45 名吸烟者为 GSTM1 阳性，4 名吸烟者为 GSTM1 缺失。GSTT1 缺失个体的高密度脂蛋白胆固醇水平（p=0.011）和血浆致动脉粥样硬化指数（p=0.01）显著降低，而共轭二烯浓度升高（p=0.01），但 GSTT1 阳性个体则没有。与 GSTT1 阳性个体相比，GSTT1 缺失个体更容易发生 DNA 损伤。由于 GSTM1 缺失型个体数量较少，所有参数均排除 GSTM1 基因型进行统计学分析。