Department of Applied Chemistry, Graduate School of Engineering, and Center for NanoBio Integration (CNBI), The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, and Japan.
Anal Chem. 2010 Jan 15;82(2):543-7. doi: 10.1021/ac9017605.
A liquid chromatography system, comprising a separation column with a width and depth of a few hundred nanometers, was fabricated on a glass microchip (femto liquid chromatography, fLC). The size of this system was approximately 10(11) times smaller than that of a conventional LC system, the flow rate was subpicoliter/minute, and the injection volume was a few hundred attoliters. The fLC system did not require packing stationary phase and was capable of separating solutes with different molecular charges (fluorescein and sulforhodamine B) that could not be separated on a conventional LC column whose surface was covered with the same functional group as that of the column of the fLC system. The fLC system represented herein overcomes limitations of conventional chromatography separation, namely, heterogeneity of the stationary phases and eddy diffusion. Scale-down of the chromatography system brought advantages not only in reduction of sample volume but also in separation efficiency. The fLC system can analyze a very small amount of sample with high efficiency and will be useful in analyzing small samples, such as single cells and synaptic clefts. fLC greatly influences and benefits various fields such as life sciences, medicine, environmental science, and manufacturing by the improvement of separation technology.
一种液相色谱系统，包括一个宽度和深度只有几百纳米的分离柱，是在玻璃微芯片上（飞秒液相色谱，fLC）制造的。该系统的尺寸大约比传统的 LC 系统小 10^11 倍，流速为亚皮升级/分钟，进样体积为几百 attoliter。fLC 系统不需要填充固定相，并且能够分离具有不同分子电荷的溶质（荧光素和磺基罗丹明 B），这些溶质在传统 LC 柱上不能分离，因为传统 LC 柱的表面覆盖着与 fLC 系统柱相同的官能团。本文所描述的 fLC 系统克服了传统色谱分离的局限性，即固定相的不均匀性和涡流扩散。色谱系统的缩小不仅在减少样品体积方面具有优势，而且在分离效率方面也具有优势。fLC 系统可以高效地分析非常少量的样品，对于分析单细胞和突触小间隙等小样本非常有用。fLC 通过改进分离技术，极大地影响和促进了生命科学、医学、环境科学和制造业等各个领域的发展。
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