甲紫与小牛胸腺 DNA 的遗传毒性相互作用研究。

Study on the genotoxic interaction of methyl violet with calf thymus DNA.

机构信息

School of Environmental Science and Engineering, Shandong University, 27# Shanda South Road, Jinan 250100, PR China.

出版信息

Appl Spectrosc. 2009 Dec;63(12):1331-5. doi: 10.1366/000370209790109085.

DOI:10.1366/000370209790109085

PMID:20030976

Abstract

By utilizing ultraviolet (UV)-visible absorption spectroscopy, circular dichroism (CD), resonance light scattering (RLS), and transmission electron microscope (TEM) techniques, the toxic interaction of methyl violet (MV) with calf thymus DNA (ctDNA) was investigated at the molecular level. The UV-visible absorption spectra results showed that MV intercalated into ctDNA base pairs at low MV concentrations, while MV was attached to the negative charged phosphate groups at higher concentrations and the binding constant (K(theta)(25 degrees C)) was 1.80 x 10(3) L mol(-1). The absorbency results showed that the genotoxic interaction of MV with ctDNA is under the electrostatic binding mode. MV had strong toxic interaction with ctDNA at 4.0 x 10(-5) mol L(-1) MV and pH 6.5. The toxic interaction of MV with ctDNA can result in the formation of massive aggregates and change of the ctDNA conformation, which showed a significant linear dose-response relationship.

摘要

利用紫外-可见吸收光谱、圆二色性（CD）、共振光散射（RLS）和透射电子显微镜（TEM）技术，在分子水平上研究了甲基紫（MV）与小牛胸腺 DNA（ctDNA）的毒性相互作用。紫外-可见吸收光谱结果表明，在低 MV 浓度下，MV 插入 ctDNA 的碱基对中，而在较高浓度下，MV 附着在带负电荷的磷酸基团上，结合常数（K（theta）（25 摄氏度））为 1.80 x 10（3）L mol（-1）。吸光度结果表明，MV 与 ctDNA 的遗传毒性相互作用属于静电结合模式。在 4.0 x 10（-5）mol L（-1）MV 和 pH 6.5 下，MV 与 ctDNA 具有很强的毒性相互作用。MV 与 ctDNA 的毒性相互作用可导致大量聚集物的形成和 ctDNA 构象的改变，这显示出显著的线性剂量反应关系。