硫化氢减轻非心脏死亡供体肾移植中缺血再灌注损伤。

Hydrogen sulphide ameliorates ischaemia-reperfusion injury in an experimental model of non-heart-beating donor kidney transplantation.

机构信息

Department of Infection, Immunity and Inflammation, Transplant Group, University of Leicester, Leicester General Hospital, Leicester LE5 4PW, UK.

出版信息

Br J Surg. 2010 Feb;97(2):202-9. doi: 10.1002/bjs.6856.

DOI:10.1002/bjs.6856

PMID:20034052

Abstract

BACKGROUND

: Therapies to alleviate ischaemia-reperfusion (IR) injury have an important role in kidney transplantation. This study used a porcine model of non-heart-beating (NHB) donor kidneys to investigate the effects of hydrogen sulphide on IR injury.

METHODS

: Porcine kidneys were subjected to 25 min of warm ischaemia and 18 h of cold storage. They were reperfused ex vivo with autologous oxygenated blood to assess renal function. A group treated with hydrogen sulphide (0.5 mmol/l) infused 10 min before and after reperfusion (n = 6) was compared with an untreated control group (n = 7).

RESULTS

: Hydrogen sulphide significantly improved renal blood flow compared with control values (mean(s.d.) area under the curve (AUC) 614.9(165.5) versus 270.3(86.7) ml per min per 100 g.h; P = 0.001) and renal function (AUC creatinine: 1640(248) versus 2328(154) micromol/l.h; P = 0.001; AUC creatinine clearance: 6.94(5.03) versus 0.96(0.32) ml per min per 100 g.h; P = 0.004). Oxidative damage was also reduced by hydrogen sulphide (urinary 8-isoprostane at 1 h of reperfusion: 478.9(237.1) versus 1605.6(632.7) pg/ml per mmol/l creatinine; P = 0.032).

CONCLUSION

: Hydrogen sulphide ameliorated the renal dysfunction associated with ischaemic damage, and has potential as a therapy against IR injury in NHB donor kidney transplantation.

摘要

背景

减轻缺血再灌注（IR）损伤的疗法在肾移植中具有重要作用。本研究使用非心脏死亡（NHB）供体肾脏的猪模型来研究硫化氢对 IR 损伤的影响。

方法

猪肾经历 25 分钟的热缺血和 18 小时的冷藏。用自体氧合血液进行体外再灌注，以评估肾功能。一组用硫化氢（0.5mmol/L）治疗，在再灌注前和再灌注后 10 分钟内输注（n=6），与未治疗的对照组（n=7）进行比较。

结果

与对照组相比，硫化氢显著改善了肾血流量（平均（标准差）曲线下面积（AUC）614.9（165.5）与 270.3（86.7）ml/min/100g.h；P=0.001）和肾功能（AUC 肌酐：1640（248）与 2328（154）µmol/L.h；P=0.001；AUC 肌酐清除率：6.94（5.03）与 0.96（0.32）ml/min/100g.h；P=0.004）。硫化氢还减少了氧化损伤（再灌注 1 小时的尿 8-异前列腺素：478.9（237.1）与 1605.6（632.7）pg/ml/mmol/L 肌酐；P=0.032）。