Detection Department, Dstl, Porton Down, Salisbury SP4 0JQ, UK.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 May 15;878(17-18):1253-6. doi: 10.1016/j.jchromb.2009.12.007. Epub 2009 Dec 5.
VX (O-ethyl-S-[2(di-isopropylamino)ethyl] methylphosphonothiolate) is a low volatility organophosphorus (OP) nerve agent and therefore the most likely route of exposure is via percutaneous absorption. Microdialysis has been used as a tool to study percutaneous poisoning by VX in the anesthetised guinea pig. A liquid chromatography tandem mass spectrometry (LC-MS-MS) method using positive electrospray ionisation (ESI) was used to quantitate VX in microdialysate samples collected from microdialysis probes, implanted into a blood vessel of anesthetised guinea pigs. The method resulted from modification of a LC-MS-MS method previously developed for the analysis of dermal microdialysates. Modification increased the sensitivity of the method, allowing quantitation of the trace levels of VX in blood microdialysates, over the range 0.002-1 ng/ml, with linear calibration. Quantitative results have been used to determine the time course of VX concentrations in the blood of guinea pigs following percutaneous poisoning.
VX（O-乙基-S-[2（二异丙基氨基）乙基]甲基膦酸硫代酯）是一种低挥发性有机磷（OP）神经毒剂，因此最有可能的暴露途径是经皮吸收。微透析已被用作研究麻醉豚鼠经皮中毒 VX 的工具。使用正电喷雾离子化（ESI）的液相色谱串联质谱（LC-MS-MS）方法，对植入麻醉豚鼠血管内的微透析探针中收集的微透析液样品中的 VX 进行定量。该方法源自先前为分析皮肤微透析液而开发的 LC-MS-MS 方法的修改。该修改提高了方法的灵敏度，允许定量检测血液微透析液中痕量 VX，范围为 0.002-1ng/ml，具有线性校准。定量结果已用于确定经皮中毒后豚鼠血液中 VX 浓度的时间过程。
