United States Department of Agriculture, Agricultural Research Service, Aquatic Animal Health Research Unit, 990 Wire Road, P.O. Box 952, Auburn, AL, 36831-0952, USA,
Fish Physiol Biochem. 2006 Mar;32(1):83-92. doi: 10.1007/s10695-006-7658-z.
The hemoglobin-beta gene of channel catfish, Ictalurus punctatus, was cloned and sequenced. Total RNA from head kidneys was isolated, reverse transcribed and amplified. The sequence of the channel catfish hemoglobin-beta gene consists of 600 nucleotides. Analysis of the nucleotide sequence reveals one open reading frame and 5'- as well as 3'-untranslated regions. The open reading frame of the sequence potentially encodes 148 amino acids with a calculated molecular mass of 16.3 kDa. The pI and charge at pH 7.0 of the deduced hemoglobin-beta protein were 7.28 and 0.47, respectively. Overall, 22 amino acid residues were conserved throughout the sequences, including His64 and His93, the sites for heme-binding. Unlike the counterpart of other common cultured fish such as Salmo salar, Oncorhynchus nerka, Oncorhynchus mykiss, Cyprinus carpio and Ctenopharyngodon idella, the hemoglobin-beta of channel catfish did not have cysteine. The amino acid sequence of channel catfish hemoglobin-beta shows 84% homology with that of Silurus asotus (both are in the order Siluriformes). However, comparison with those of other fish species shows homology ranging from 53 to 68%. Structural analysis by the 3D-PSSM program displays that channel catfish hemoglobin-beta has eight alpha-helices, A-H.
斑点叉尾鮰血红蛋白-β基因的克隆与测序。从头肾中分离总 RNA，反转录并扩增。斑点叉尾鮰血红蛋白-β基因序列由 600 个核苷酸组成。核苷酸序列分析揭示了一个开放阅读框和 5' 以及 3' 非翻译区。该序列的开放阅读框可能编码 148 个氨基酸，计算分子量为 16.3 kDa。推导的血红蛋白-β蛋白的等电点和 pH7.0 时的电荷分别为 7.28 和 0.47。总体而言，整个序列中有 22 个氨基酸残基保守，包括血红素结合位点 His64 和 His93。与其他常见养殖鱼类（如 Salmo salar、Oncorhynchus nerka、Oncorhynchus mykiss、Cyprinus carpio 和 Ctenopharyngodon idella）的血红蛋白-β不同，斑点叉尾鮰的血红蛋白-β没有半胱氨酸。斑点叉尾鮰血红蛋白-β的氨基酸序列与 Silurus asotus（均属于 Siluriformes 目）的同源性为 84%。然而，与其他鱼类物种的比较显示同源性范围为 53% 至 68%。3D-PSSM 程序的结构分析显示，斑点叉尾鮰血红蛋白-β具有 8 个α-螺旋，A-H。
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