Department of Environmental Science and Engineering, Guru Jambheshwar University of Science and Technology, Hisar 125001, Haryana, India.
J Hazard Mater. 2010 Apr 15;176(1-3):1079-82. doi: 10.1016/j.jhazmat.2009.10.120. Epub 2009 Nov 10.
Laccase is among the major enzymes which plays an important role in ligninolytic system of fungi. Laccase production by Aspergillus heteromorphus was studied using anaerobically treated distillery spent wash (ADSW) and lignocellulosic biomass. Lignocellulosic biomass (rice straw, wheat straw and sugarcane bagasse) generated during biomass processing leads to solid waste and distillery spent wash is unwanted liquid waste produced by distilleries, both causes environmental pollution. Two mineral media and anaerobically treated distillery spent wash medium was tested for laccase production. Enzyme production in various media and in presence and absence of lignocellulosic biomass supplements showed that anaerobically treated distillery spent wash medium was a better laccase inducer medium than the mineral media. Addition of lignocellulosic biomass enhances laccase production and highest laccase activity was obtained in 5% anaerobically treated distillery spent wash medium with rice straw.
漆酶是真菌木质素降解系统中的主要酶类之一。本研究采用厌氧处理的酿酒废醪（ADSW）和木质纤维素生物质来研究杂色曲霉中产漆酶的情况。生物质加工过程中产生的木质纤维素生物质（稻草、麦秆和甘蔗渣）会产生固体废物，而酿酒厂产生的酿酒废醪则是不需要的液体废物，两者都会造成环境污染。本研究测试了两种矿物培养基和厌氧处理的酿酒废醪培养基用于产漆酶。在各种培养基以及添加和不添加木质纤维素生物质的情况下进行酶生产，结果表明，厌氧处理的酿酒废醪培养基比矿物培养基更适合作为漆酶诱导培养基。添加木质纤维素生物质可提高漆酶的产量，在 5%厌氧处理的酿酒废醪培养基中添加稻草时，漆酶的活性最高。
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