Yağci Ramazan, Akçali Alper, Yağci Server, Konno Tsunetada, Ishiko Hiroaki, Duman Sunay, Ozkaya Etem
Fatih University, Medical School, Department of Ophthalmology, Ankara, Turkey.
Eur J Ophthalmol. 2010 Jul-Aug;20(4):669-74. doi: 10.1177/112067211002000404.
The aim of the study was isolation of adenoviruses by cell culture and identification using polymerase chain reaction (PCR) and phylogenetic analyses in patients clinically diagnosed with viral conjunctivitis in Ankara, Turkey.
Conjunctival swabs from 34 patients with acute conjunctivitis were tested using cell culture isolation and PCR for adenovirus detection. PCR-positive samples were sequenced and typed.
The positive results of adenovirus were 26.5% (9 of 34) by the PCR method and 20.6% by culture isolation. Nine samples positive at PCR were identified by phylogenetic analyses as human adenovirus 8 (HAdV-8) (4 of 9), HAdV-3 (3 of 9), HAdV-4 (1 of 9), and HAdV-B (1 of 9).
Our study showed types of adenoviruses in patients with ocular infection that occurred in this region of Turkey for the first time. Furthermore, sequence-based typing method is an efficient, accurate, and rapid means of diagnosis and typing of the adenovirus and has significant clinical and epidemiologic implications. HAdV-8 was major type for acute conjunctivitis in Ankara, Turkey. Further studies are required to reveal the major types of HAdVs that cause ocular diseases in this region of the world.
本研究旨在通过细胞培养分离腺病毒，并利用聚合酶链反应（PCR）和系统发育分析对土耳其安卡拉临床诊断为病毒性结膜炎的患者进行鉴定。
对34例急性结膜炎患者的结膜拭子进行细胞培养分离和PCR检测以检测腺病毒。对PCR阳性样本进行测序和分型。
PCR法检测腺病毒的阳性率为26.5%（34例中的9例），培养分离法的阳性率为20.6%。通过系统发育分析，9例PCR阳性样本被鉴定为人腺病毒8型（HAdV-8）（9例中的4例）、HAdV-3型（9例中的3例）、HAdV-4型（9例中的1例）和HAdV-B型（9例中的1例）。
我们的研究首次显示了在土耳其该地区发生的眼部感染患者中的腺病毒类型。此外，基于序列的分型方法是一种高效、准确且快速的腺病毒诊断和分型手段，具有重要的临床和流行病学意义。HAdV-8是土耳其安卡拉急性结膜炎的主要类型。需要进一步研究以揭示在世界该地区引起眼部疾病的主要HAdV类型。
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